Temporal dissection of Bax-induced events leading to fission of the single mitochondrion in Trypanosoma brucei
- Crausaz Esseiva, Anne Zoology, Department of Biology, University of Fribourg, Switzerland
- Chanez, Anne-Laure Zoology, Department of Biology, University of Fribourg, Switzerland
- Bochud-Allemann, Natacha Zoology, Department of Biology, University of Fribourg, Switzerland
- Martinou, Jean-Claude Departement de Biologie Cellulaire, Sciences III, Genève, Switzerland
- Hemphill, Andrew Institute of Parasitology, University of Bern, Switzerland
- Schneider, André Zoology, Department of Biology, University of Fribourg, Switzerland
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13.02.2004
Published in:
- EMBO reports. - 2004, vol. 5, no. 3, p. 268-273
English
The protozoan Trypanosoma brucei has a single mitochondrion and lacks an apoptotic machinery. Here we show that expression of the proapoptotic protein Bax in T. brucei causes the release of cytochrome c, the depolarization of the mitochondrial membrane potential and mitochondrial fission. However, in contrast to mammalian cells, the three events are temporally well separated. The release of cytochrome c from the intermembrane space precedes mitochondrial fission, showing that it does not depend on mitochondrial fragmentation. Furthermore, halting Bax expression allows some cells to recover even after mitochondrial fission, the last recorded event, went to completion, indicating that all three Bax-induced events are, in principle, reversible.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 4143
- DOI 10.1038/sj.embor.7400095
- Persistent URL
- https://folia.unifr.ch/unifr/documents/299564
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