Back
Full-text
Journal article

Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity.

  • Fischer M Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Dübendorf, Switzerland.
  • Belanger SE The Procter & Gamble Company, Global Product Stewardship, Mason, Ohio 45040, USA.
  • Berckmans P VITO-ABS team, Department of Environmental Risk and Health, Flemish Institute for Technological Research, 2400 Mol, Belgium.
  • Bernhard MJ The Procter & Gamble Company, Global Product Stewardship, Mason, Ohio 45040, USA.
  • Bláha L RECETOX, Faculty of Science, Masaryk University, 625 00 Brno-Bohunice, Czech Republic.
  • Coman Schmid DE Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Dübendorf, Switzerland.
  • Dyer SD The Procter & Gamble Company, Global Product Stewardship, Mason, Ohio 45040, USA.
  • Haupt T Givaudan Schweiz AG, Department of In vitro Molecular Screening, 8310 Kemptthal, Switzerland.
  • Hermens JLM IRAS, The Institute for Risk Assessment Sciences, Toxicology Division, Utrecht University, 3584 CL Utrecht, The Netherlands.
  • Hultman MT Norwegian Institute for Water Research (NIVA), Section for ecotoxicology, 0349 Oslo, Norway.
  • Laue H Givaudan Schweiz AG, Department of In vitro Molecular Screening, 8310 Kemptthal, Switzerland.
  • Lillicrap A Norwegian Institute for Water Research (NIVA), Section for ecotoxicology, 0349 Oslo, Norway.
  • Mlnaříková M RECETOX, Faculty of Science, Masaryk University, 625 00 Brno-Bohunice, Czech Republic.
  • Natsch A Givaudan Schweiz AG, Department of In vitro Molecular Screening, 8310 Kemptthal, Switzerland.
  • Novák J RECETOX, Faculty of Science, Masaryk University, 625 00 Brno-Bohunice, Czech Republic.
  • Sinnige TL IRAS, The Institute for Risk Assessment Sciences, Toxicology Division, Utrecht University, 3584 CL Utrecht, The Netherlands.
  • Tollefsen KE Norwegian Institute for Water Research (NIVA), Section for ecotoxicology, 0349 Oslo, Norway.
  • von Niederhäusern V Givaudan Schweiz AG, Department of In vitro Molecular Screening, 8310 Kemptthal, Switzerland.
  • Witters H VITO-ABS team, Department of Environmental Risk and Health, Flemish Institute for Technological Research, 2400 Mol, Belgium.
  • Županič A Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Dübendorf, Switzerland.
  • Schirmer K Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, 8600 Dübendorf, Switzerland.
Show more…
  • 2019-03-03
Published in:
  • Toxicological sciences : an official journal of the Society of Toxicology. - 2019
in vitro alternatives
round-robin study
validation
Aniline Compounds
Animals
Cell Line
Cell Survival
Dose-Response Relationship, Drug
Laboratories
Oncorhynchus mykiss
Reproducibility of Results
Toxicity Tests, Acute
English Predicting fish acute toxicity of chemicals in vitro is an attractive alternative method to the conventional approach using juvenile and adult fish. The rainbow trout (Oncorhynchus mykiss) cell line assay with RTgill-W1 cells has been designed for this purpose. It quantifies cell viability using fluorescent measurements for metabolic activity, cell- and lysosomal-membrane integrity on the same set of cells. Results from over 70 organic chemicals attest to the high predictive capacity of this test. We here report on the repeatability (intralaboratory variability) and reproducibility (interlaboratory variability) of the RTgill-W1 cell line assay in a round-robin study focusing on 6 test chemicals involving 6 laboratories from the industrial and academic sector. All participating laboratories were able to establish the assay according to preset quality criteria even though, apart from the lead laboratory, none had previously worked with the RTgill-W1 cell line. Concentration-response modeling, based on either nominal or geometric mean-derived measured concentrations, yielded effect concentrations (EC50) that spanned approximately 4 orders of magnitude over the chemical range, covering all fish acute toxicity categories. Coefficients of variation for intralaboratory and interlaboratory variability for the average of the 3 fluorescent cell viability measurements were 15.5% and 30.8%, respectively, which is comparable to other fish-derived, small-scale bioassays. This study therefore underlines the robustness of the RTgill-W1 cell line assay and its accurate performance when carried out by operators in different laboratory settings.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/94919
Statistics
Document views: 60 File downloads:
  • Full-text: 0