Air-Liquid Interface In Vitro Models for Respiratory Toxicology Research: Consensus Workshop and Recommendations.
- Lacroix G Chronic Risks Division, Institut National de l'Environnement Industriel et des RISques, Verneuil-en-Halatte, France.
- Koch W In Vitro und Mechanistische Toxikologie, Fraunhofer ITEM, Hannover, Germany.
- Ritter D In Vitro und Mechanistische Toxikologie, Fraunhofer ITEM, Hannover, Germany.
- Gutleb AC Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, Esch-sur-Alzette, Luxembourg.
- Larsen ST Inhalation Toxicology Group, National Research Centre for the Working Environment, Copenhagen, Denmark.
- Loret T Chronic Risks Division, Institut National de l'Environnement Industriel et des RISques, Verneuil-en-Halatte, France.
- Zanetti F Systems Toxicology Department, Philip Morris International R&D, Neuchâtel, Switzerland.
- Constant S Epithelix Sarl, Plan-les-Outes, Switzerland.
- Chortarea S BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
- Rothen-Rutishauser B BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
- Hiemstra PS Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands.
- Frejafon E Chronic Risks Division, Institut National de l'Environnement Industriel et des RISques, Verneuil-en-Halatte, France.
- Hubert P Chronic Risks Division, Institut National de l'Environnement Industriel et des RISques, Verneuil-en-Halatte, France.
- Gribaldo L Directorate F-Health, Consumers and Reference Materials Chemicals Safety and Alternative Methods Unit (F.3), EURL ECVAM, JRC, Ispra, Italy.
- Kearns P Environment, Health and Safety Division, OECD, Paris, France.
- Aublant JM European Affairs and Standardization, Laboratoire National de Métrologie et d'Essais, Paris, France.
- Diabaté S Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany.
- Weiss C Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany.
- de Groot A Toxicological and Environmental Risk Assessment (TERA) Department, Solvay, Brussels, Belgium.
- Kooter I Department of Circular Environment and Environment (CEE), TNO, Utrecht, The Netherlands.
- 2020-09-21
Published in:
- Applied in vitro toxicology. - 2018
English
In vitro air-liquid interface (ALI) cell culture models can potentially be used to assess inhalation toxicology endpoints and are usually considered, in terms of relevancy, between classic (i.e., submerged) in vitro models and animal-based models. In some situations that need to be clearly defined, ALI methods may represent a complement or an alternative option to in vivo experimentations or classic in vitro methods. However, it is clear that many different approaches exist and that only very limited validation studies have been carried out to date. This means comparison of data from different methods is difficult and available methods are currently not suitable for use in regulatory assessments. This is despite inhalation toxicology being a priority area for many governmental organizations. In this setting, a 1-day workshop on ALI in vitro models for respiratory toxicology research was organized in Paris in March 2016 to assess the situation and to discuss what might be possible in terms of validation studies. The workshop was attended by major parties in Europe and brought together more than 60 representatives from various academic, commercial, and regulatory organizations. Following plenary, oral, and poster presentations, an expert panel was convened to lead a discussion on possible approaches to validation studies for ALI inhalation models. A series of recommendations were made and the outcomes of the workshop are reported.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1089/aivt.2017.0034
- PMID 32953944
- Persistent URL
- https://folia.unifr.ch/global/documents/80616
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