Transitional B cells commit to marginal zone B cell fate by Taok3-mediated surface expression of ADAM10.
- Hammad H VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Vanderkerken M VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Pouliot P VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Deswarte K VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Toussaint W VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Vergote K VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Vandersarren L VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Janssens S VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Ramou I VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Savvides SN VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Haigh JJ VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- Hendriks R Department of Pulmonary Medicine, Erasmus Medical Center, Rotterdam, the Netherlands.
- Kopf M Institute for Molecular Health Sciences, ETH, Zürich, Switzerland.
- Craessaerts K VIB Center for Brain and Disease, VIB, Leuven, Belgium.
- de Strooper B VIB Center for Brain and Disease, VIB, Leuven, Belgium.
- Kearney JF Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
- Conrad DH Center for Clinical and Translational Research, Virginia Commonwealth University, Richmond, Virginia, USA.
- Lambrecht BN VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
- 2017-01-10
Published in:
- Nature immunology. - 2017
ADAM10 Protein
Adaptive Immunity
Amyloid Precursor Protein Secretases
Animals
B-Lymphocytes
Cell Differentiation
Cell Lineage
Cells, Cultured
Clonal Selection, Antigen-Mediated
Gene Expression Regulation
Germinal Center
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Mice
Mice, Inbred C57BL
Mice, Knockout
Protein-Serine-Threonine Kinases
Receptor, Notch2
Receptors, Antigen, B-Cell
Signal Transduction
English
Notch2 and B cell antigen receptor (BCR) signaling determine whether transitional B cells become marginal zone B (MZB) or follicular B (FoB) cells in the spleen, but it is unknown how these pathways are related. We generated Taok3-/- mice, lacking the serine/threonine kinase Taok3, and found cell-intrinsic defects in the development of MZB but not FoB cells. Type 1 transitional (T1) B cells required Taok3 to rapidly respond to ligation by the Notch ligand Delta-like 1. BCR ligation by endogenous or exogenous ligands induced the surface expression of the metalloproteinase ADAM10 on T1 B cells in a Taok3-dependent manner. T1 B cells expressing surface ADAM10 were committed to becoming MZB cells in vivo, whereas T1 B cells lacking expression of ADAM10 were not. Thus, during positive selection in the spleen, BCR signaling causes immature T1 B cells to become receptive to Notch ligands via Taok3-mediated surface expression of ADAM10.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1038/ni.3657
- PMID 28068307
- Persistent URL
- https://folia.unifr.ch/global/documents/77558
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