Super-resolution microscopy demystified.
- Schermelleh L Micron Oxford Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, Oxford, UK. lothar.schermelleh@bioch.ox.ac.uk.
- Ferrand A Imaging Core Facility, Biozentrum, University of Basel, Basel, Switzerland.
- Huser T Biomolecular Photonics, Department of Physics, University of Bielefeld, Bielefeld, Germany.
- Eggeling C MRC Human Immunology Unit and Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
- Sauer M Department of Biotechnology & Biophysics, Biocenter, Julius Maximilian University of Würzburg, Würzburg, Germany.
- Biehlmaier O Imaging Core Facility, Biozentrum, University of Basel, Basel, Switzerland.
- Drummen GPC Advanced Bio-Imaging Program, Bio&Nano Solutions‒LAB3BIO, Bielefeld, Germany. gpcdrummen@bionano-solutions.de.
- 2019-01-04
Published in:
- Nature cell biology. - 2019
Animals
Cell Biology
Humans
Imaging, Three-Dimensional
Microscopy, Fluorescence
Molecular Biology
Reproducibility of Results
English
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1038/s41556-018-0251-8
- PMID 30602772
- Persistent URL
- https://folia.unifr.ch/global/documents/73204
Statistics
Document views: 134
File downloads:
- Full-text: 0