Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis.
- Méric G The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
- Mageiros L The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
- Pensar J Department of Mathematics and Statistics, University of Helsinki, Helsinki, 00100, Finland.
- Laabei M The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
- Yahara K Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan.
- Pascoe B The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
- Kittiwan N Integrative Research Centre for Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
- Tadee P Graduate School, Maejo University, Chiang Mai, 50290, Thailand.
- Post V AO Research Institute Davos, Davos, 7270, Switzerland.
- Lamble S Wellcome Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, UK.
- Bowden R Wellcome Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, UK.
- Bray JE Department of Zoology, University of Oxford, Oxford, OX1 3SZ, UK.
- Morgenstern M Department of Orthopaedic Surgery and Traumatology, University Hospital Basel, Basel, 4031, Switzerland.
- Jolley KA Department of Zoology, University of Oxford, Oxford, OX1 3SZ, UK.
- Maiden MCJ Department of Zoology, University of Oxford, Oxford, OX1 3SZ, UK.
- Feil EJ The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
- Didelot X Department of Infectious Disease Epidemiology, Imperial College, London, SW7 2AZ, UK.
- Miragaia M Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, 2775-412, Portugal.
- de Lencastre H Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, 2775-412, Portugal.
- Moriarty TF AO Research Institute Davos, Davos, 7270, Switzerland.
- Rohde H Institut für Medizinische Mikrobiologie, Virologie & Hygiene, Universität Hamburg, Hamburg, 20246, Germany.
- Massey R The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
- Mack D Bioscientia Labor Ingelheim, Institut für Medizinische Diagnostik GmbH, Ingelheim, 55218, Germany.
- Corander J Department of Mathematics and Statistics, University of Helsinki, Helsinki, 00100, Finland.
- Sheppard SK The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, BA2 7AY, UK. s.k.sheppard@bath.ac.uk.
- 2018-11-30
Published in:
- Nature communications. - 2018
Genome, Bacterial
Genome-Wide Association Study
Genotype
Humans
Interleukin-8
Skin Diseases
Staphylococcal Infections
Staphylococcus epidermidis
English
Some of the most common infectious diseases are caused by bacteria that naturally colonise humans asymptomatically. Combating these opportunistic pathogens requires an understanding of the traits that differentiate infecting strains from harmless relatives. Staphylococcus epidermidis is carried asymptomatically on the skin and mucous membranes of virtually all humans but is a major cause of nosocomial infection associated with invasive procedures. Here we address the underlying evolutionary mechanisms of opportunistic pathogenicity by combining pangenome-wide association studies and laboratory microbiology to compare S. epidermidis from bloodstream and wound infections and asymptomatic carriage. We identify 61 genes containing infection-associated genetic elements (k-mers) that correlate with in vitro variation in known pathogenicity traits (biofilm formation, cell toxicity, interleukin-8 production, methicillin resistance). Horizontal gene transfer spreads these elements, allowing divergent clones to cause infection. Finally, Random Forest model prediction of disease status (carriage vs. infection) identifies pathogenicity elements in 415 S. epidermidis isolates with 80% accuracy, demonstrating the potential for identifying risk genotypes pre-operatively.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1038/s41467-018-07368-7
- PMID 30487573
- Persistent URL
- https://folia.unifr.ch/global/documents/73200
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