Histone chaperone ASF1 cooperates with the Brahma chromatin-remodelling machinery.
- Moshkin YM Department of Zoology and Animal Biology, University of Geneva, 1211 Geneva 4, Switzerland.
- Armstrong JA
- Maeda RK
- Tamkun JW
- Verrijzer P
- Kennison JA
- Karch F
- 2002-10-17
Published in:
- Genes & development. - 2002
Animals
Cell Cycle Proteins
Chromosomal Proteins, Non-Histone
Crosses, Genetic
Drosophila Proteins
Drosophila melanogaster
Eye Color
Female
Gene Deletion
Gene Silencing
Heterozygote
In Vitro Techniques
Male
Molecular Chaperones
Mutation
Nucleosomes
Protein Transport
Transcription Factors
English
De novo chromatin assembly into regularly spaced nucleosomal arrays is essential for eukaryotic genome maintenance and inheritance. The Anti-Silencing Function 1 protein (ASF1) has been shown to be a histone chaperone, participating in DNA-replication-coupled nucleosome assembly. We show that mutations in the Drosophila asf1 gene derepress silencing at heterochromatin and that the ASF1 protein has a cell cycle-specific nuclear and cytoplasmic localization. Furthermore, using both genetic and biochemical methods, we demonstrate that ASF1 interacts with the Brahma (SWI/SNF) chromatin-remodelling complex. These findings suggest that ASF1 plays a crucial role in both chromatin assembly and SWI/SNF-mediated chromatin remodelling.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1101/gad.231202
- PMID 12381660
- Persistent URL
- https://folia.unifr.ch/global/documents/65156
Statistics
Document views: 22
File downloads:
- Full-text: 0