Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry-Based Assays.
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Hoofnagle AN
University of Washington, Seattle, WA; ahoof@u.washington.edu apaulovi@fhcrc.org.
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Whiteaker JR
Fred Hutchinson Cancer Research Center, Seattle, WA;
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Carr SA
Broad Institute, Cambridge, MA;
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Kuhn E
Broad Institute, Cambridge, MA;
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Liu T
Pacific Northwest National Laboratory, Richland, WA;
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Massoni SA
New England Peptide, Inc., Gardner, MA;
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Thomas SN
Johns Hopkins University, Baltimore, MD;
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Townsend RR
Washington University, St Louis, MO;
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Zimmerman LJ
Vanderbilt University, Nashville, TN;
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Boja E
National Cancer Institute, Bethesda, MD;
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Chen J
Johns Hopkins University, Baltimore, MD;
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Crimmins DL
Washington University, St Louis, MO;
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Davies SR
Washington University, St Louis, MO;
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Gao Y
Pacific Northwest National Laboratory, Richland, WA;
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Hiltke TR
National Cancer Institute, Bethesda, MD;
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Ketchum KA
ESAC, Inc., Rockville, MD;
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Kinsinger CR
National Cancer Institute, Bethesda, MD;
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Mesri M
National Cancer Institute, Bethesda, MD;
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Meyer MR
Washington University, St Louis, MO;
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Qian WJ
Pacific Northwest National Laboratory, Richland, WA;
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Schoenherr RM
Fred Hutchinson Cancer Research Center, Seattle, WA;
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Scott MG
Washington University, St Louis, MO;
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Shi T
Pacific Northwest National Laboratory, Richland, WA;
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Whiteley GR
Frederick National Laboratory for Cancer Research, Frederick, MD;
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Wrobel JA
University of North Carolina School of Medicine, Chapel Hill, NC;
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Wu C
Pacific Northwest National Laboratory, Richland, WA;
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Ackermann BL
Eli Lilly and Company, Indianapolis, IN;
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Aebersold R
Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland;
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Barnidge DR
Mayo Clinic College of Medicine, Rochester, MN;
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Bunk DM
NIST, Gaithersburg, MD;
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Clarke N
Quest Diagnostics, San Juan Capistrano, CA;
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Fishman JB
21st Century Biochemicals, Inc., Marlborough, MA;
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Grant RP
Laboratory Corporation of America Holdings, Inc., Burlington, NC;
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Kusebauch U
Institute for Systems Biology, Seattle, WA;
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Kushnir MM
University of Utah and ARUP Laboratories, Salt Lake City, UT;
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Lowenthal MS
NIST, Gaithersburg, MD;
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Moritz RL
Institute for Systems Biology, Seattle, WA;
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Neubert H
Pfizer, Inc., Andover, MA;
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Patterson SD
Gilead Sciences, Inc., Foster City, CA;
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Rockwood AL
University of Utah and ARUP Laboratories, Salt Lake City, UT;
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Rogers J
Thermo Fisher Scientific, Rockford, IL;
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Singh RJ
Mayo Clinic College of Medicine, Rochester, MN;
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Van Eyk JE
Cedars Sinai Medical Center, Los Angeles, CA;
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Wong SH
Wake Forest School of Medicine, Winston-Salem, NC;
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Zhang S
Enanta Pharmaceuticals, Watertown, MA;
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Chan DW
Johns Hopkins University, Baltimore, MD;
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Chen X
University of North Carolina School of Medicine, Chapel Hill, NC;
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Ellis MJ
Baylor College of Medicine, Houston, TX.
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Liebler DC
Vanderbilt University, Nashville, TN;
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Rodland KD
Pacific Northwest National Laboratory, Richland, WA;
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Rodriguez H
National Cancer Institute, Bethesda, MD;
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Smith RD
Pacific Northwest National Laboratory, Richland, WA;
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Zhang Z
Johns Hopkins University, Baltimore, MD;
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Zhang H
Johns Hopkins University, Baltimore, MD;
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Paulovich AG
Fred Hutchinson Cancer Research Center, Seattle, WA; ahoof@u.washington.edu apaulovi@fhcrc.org.
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Published in:
- Clinical chemistry. - 2016
English
BACKGROUND
For many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotope-labeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays.
CONTENT
The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling, as well as approaches to the interpretation of the data generated by mass spectrometric protein assays. Additionally, the importance of carefully characterized reference materials-in particular, peptide standards for the improved concordance of amino acid analysis methods across the industry-is highlighted. The alignment of practices around the use of peptides and the transparency of sample preparation protocols should allow for the harmonization of peptide and protein quantification in research and clinical care.
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Language
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Open access status
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bronze
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Persistent URL
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https://folia.unifr.ch/global/documents/64599
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