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Journal article

Intestinal intraepithelial lymphocytes exert potent protective cytotoxic activity during an acute virus infection.

  • 2000-02-05
Published in:
  • Journal of immunology (Baltimore, Md. : 1950). - 2000
Acute Disease
Animals
CD8 Antigens
CD8-Positive T-Lymphocytes
Cells, Cultured
Cytotoxicity, Immunologic
Dendritic Cells
Granzymes
Intestinal Mucosa
Kinetics
Lymphocyte Activation
Lymphocyte Subsets
Lymphocytic Choriomeningitis
Lymphocytic choriomeningitis virus
Macrophages
Membrane Glycoproteins
Mice
Mice, Inbred C57BL
Perforin
Pore Forming Cytotoxic Proteins
RNA, Messenger
Receptors, Antigen, T-Cell, alpha-beta
Serine Endopeptidases
Spleen
Up-Regulation
Virus Replication
English After systemic infection of mice with 104 PFU of lymphocytic choriomeningitis virus (LCMV), infected cells are detected simultaneously in various organs, including spleen and intestinal mucosa. Most notably, virus-infected cells are also present among CD11c+ dendritic cells in the subepithelial area of the small intestinal mucosa. Some of these virus-infected cells are in close spatial association with intestinal intraepithelial lymphocytes (IEL). Therefore, we compared virus-specific cytotoxic activity of CD8 splenocytes with that of IEL subsets. While ex vivo isolated TCRalphabeta+CD8alphaalpha+ IEL exert only minimal virus-specific cytotoxicity, maximum specific killing mediated by TCRalphabeta+CD8alphabeta+ IEL on day 8 postinfection exceeds maximum cytotoxic activity observed with CD8 splenocytes when assessed in vitro. Maximum cytotoxic activity of IEL is preceded by peak perforin and granzyme B mRNA expression in IEL around day 6 postinfection, suggesting a recent activation in situ. The antivirus cytotoxicity of in vivo primed IEL is further demonstrated by the protection from virus production in the spleen of mice infected with LCMV 10 h before adoptive cell transfer. These data indicate a potent priming of LCMV-specific IEL in situ after systemic LCMV infection and suggest that cytotoxic IEL markedly contribute to the elimination of virus-infected cells in the intestinal mucosa.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/63861
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