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PLASMINOGEN ACTIVATOR INHIBITOR (PAI) ACTIVITY AS WELL AS PAI-1 AND PAI-2 ANTIGEN IN HEALTHY INDIVIDUALS AND HOSPITALIZED PATIENTS
Journal article

PLASMINOGEN ACTIVATOR INHIBITOR (PAI) ACTIVITY AS WELL AS PAI-1 AND PAI-2 ANTIGEN IN HEALTHY INDIVIDUALS AND HOSPITALIZED PATIENTS

  • Kruithof, E K O Laboratoire central d'hematologie, CHUV, Lausanne, Switzerland
  • Nicoloso, G Laboratoire central d'hematologie, CHUV, Lausanne, Switzerland
  • Gudinchet, A Laboratoire central d'hematologie, CHUV, Lausanne, Switzerland
  • Bachmann, F Laboratoire central d'hematologie, CHUV, Lausanne, Switzerland
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  • 2018-8-23
Published in:
  • XIth International Congress on Thrombosis and Haemostasis. - Schattauer GmbH. - 1987
English The activity of plasminogen activators (PA) is regulated in part by PA-inhibitors. Two distinct PAIs have been identified: PAI-1 and PAI-2. To be able to measure these proteins specifically we have purified PAI-1 and PAI-2, raised antisera and developed specific radioimmunoassays (RIAs), with detection limits in plasma of 10 and 15 ng/ml, respectively.In a population of over 350 individuals, PAI activity correlated well with PAI-1 :Ag (r=0.699), but not with PAI-2:Ag (r=0.070). In healthy individuals PAI-1 concentrations varied considerably (between 10 and 85 ng/ml, median 29 ng/ml). During venous occlusion (VO) applied in 40 healthy individuals, average PAI-1 in plasma increased fran 27 before, to 43 and 59 ng/ml after 10 and 20 min VO (p <0.001, Wilcoxon test). Overall fibrinolytic activity increased 15 fold, and t-PA:Ag levels eightfold. Increased PAI activity and PAI-1 antigen levels with respect to controls were found in patients with cardiovascular or thromboembolic disease, malignancies, hepatic insufficiency, after major trauma and in the postoperative period. This wide spectrum of pathologies with increased PAI-1 levels supports previous suggestions that PAI-1 behaves as an acute phase reactant. In patients undergoing extracorporeal circulation PAI activity and PAI-1 antigen were measured before and 1 h, 1 d and 7 d after the operation. Highest values of activity and antigen were observed 1 h postoperatively. These values remained elevated the day after the operation, but returned to preoperative levels within 7 days.PAI-2 antigen concentrations were at or below the detection limit in all controls and the majority of patients. Very high levels of PAI-2 (above 30 ng/ml) were only observed in pregnant women and in patients with hepatocellular carcinoma.The specific measurement of PAIs thus show increased levels in a variety of clinical conditions. The availability of specific RIAs permits to conduct prospective studies and to evaluate to what degree elevated PAI-1 and PAI-2 concentrations are correlated with the stage of the disease, its prognosis and the risk to develop thromboembolic complications.
Language
  • English
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closed
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Persistent URL
https://folia.unifr.ch/global/documents/54772
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