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Journal article

9-cis-retinoic acid is a natural antagonist for the retinoic acid receptor response pathway.

  • 1993-10-15
Published in:
  • The Biochemical journal. - 1993
Animals
Cells, Cultured
Chlorocebus aethiops
DNA
Drosophila
Isomerism
Protein Biosynthesis
Receptors, Retinoic Acid
Transcriptional Activation
Tretinoin
English The pleiotropic activities of retinoids are mediated by two types of nuclear receptors, the retinoic acid receptors (RARs) and the retinoid X receptors (RXRs). All-trans-retinoic acid (RA) transcriptionally activates RARs, but not RXRs, whereas its natural stereoisomer, 9-cis-RA, is the ligand for RXRs. Here, we demonstrate that 9-cis-RA did not transcriptionally activate RARs, whereas in the presence of all-trans-RA the transactivation of RARs was inhibited in a dose-dependent manner by 9-cis-RA. RAR homodimer complexes were destabilized in vitro in the presence of 9-cis-RA. This suggests that 9-cis-RA may be a natural antagonist of all-trans-RA for binding to RAR complexes. The levels of 9-cis-RA may determine by which pathway the transcription of retinoid-responsive genes is modulated.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/5117
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