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Rapid Morphological and Cytoskeletal Response to Microgravity in Human Primary Macrophages.

  • Thiel CS Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. cora.thiel@uzh.ch.
  • Tauber S Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. svantje.tauber@uzh.ch.
  • Lauber B Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. beatrice.lauber@anatomy.uzh.ch.
  • Polzer J Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. jennifer.polzer@uzh.ch.
  • Seebacher C TILL I.D. GmbH, Am Klopferspitz 19a, 82152 Martinsried, Germany. seebacher@till-id.com.
  • Uhl R TILL I.D. GmbH, Am Klopferspitz 19a, 82152 Martinsried, Germany. rainer_uhl@me.com.
  • Neelam S National Aeronautics and Space Administration (NASA), ISS Utilization and Life Sciences Division, Kennedy Space Center, Cape Canaveral, FL 32899, USA. neelamsrjn@gmail.com.
  • Zhang Y National Aeronautics and Space Administration (NASA), ISS Utilization and Life Sciences Division, Kennedy Space Center, Cape Canaveral, FL 32899, USA. ye.zhang-1@nasa.gov.
  • Levine H National Aeronautics and Space Administration (NASA), ISS Utilization and Life Sciences Division, Kennedy Space Center, Cape Canaveral, FL 32899, USA. howard.g.levine@nasa.gov.
  • Ullrich O Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. oliver.ullrich@uzh.ch.
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  • 2019-05-18
Published in:
  • International journal of molecular sciences. - 2019
cytoskeleton
immune cells
live cell imaging
microgravity
nucleus
suborbital rocket
Actin Cytoskeleton
Actins
Cell Line
Cell Nucleus
Cytoplasm
Cytoskeleton
Humans
Lysosomes
Macrophages
Microscopy, Confocal
Microscopy, Fluorescence
Monocytes
Space Flight
Weightlessness
English The FLUMIAS (Fluorescence-Microscopic Analyses System for Life-Cell-Imaging in Space) confocal laser spinning disk fluorescence microscope represents a new imaging capability for live cell imaging experiments on suborbital ballistic rocket missions. During the second pioneer mission of this microscope system on the TEXUS-54 suborbital rocket flight, we developed and performed a live imaging experiment with primary human macrophages. We simultaneously imaged four different cellular structures (nucleus, cytoplasm, lysosomes, actin cytoskeleton) by using four different live cell dyes (Nuclear Violet, Calcein, LysoBrite, SiR-actin) and laser wavelengths (405, 488, 561, and 642 nm), and investigated the cellular morphology in microgravity (10-4 to 10-5 g) over a period of about six minutes compared to 1 g controls. For live imaging of the cytoskeleton during spaceflight, we combined confocal laser microscopy with the SiR-actin probe, a fluorogenic silicon-rhodamine (SiR) conjugated jasplakinolide probe that binds to F-actin and displays minimal toxicity. We determined changes in 3D cell volume and surface, nuclear volume and in the actin cytoskeleton, which responded rapidly to the microgravity environment with a significant reduction of SiR-actin fluorescence after 4-19 s microgravity, and adapted subsequently until 126-151 s microgravity. We conclude that microgravity induces geometric cellular changes and rapid response and adaptation of the potential gravity-transducing cytoskeleton in primary human macrophages.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/48061
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