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In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering.
Journal article

In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering.

  • Peloso A Department of Clinical, Surgical, Diagnostic and Paediatric Sciences, University of Pavia, Pavia, Italy. andreapeloso@hotmail.it.
  • Citro A San Raffaele Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  • Corradetti V IRCCS Policlinico San Matteo, Unit of Nephrology, Dialysis and Transplantation, Pavia, Italy.
  • Brambilla S University of Pavia, Pavia, Italy.
  • Oldani G Divisions of Abdominal and Transplantation Surgery, Hepato-Pancreato-Biliary Centre, Department of Surgery, Faculty of Medicine, University Hospital of Geneva, University of Geneva, Geneva, Switzerland.
  • Calabrese F Department of General Surgery, IRCCS Policlinico San Matteo, Pavia, Italy.
  • Dominioni T Department of General Surgery, IRCCS Policlinico San Matteo, Pavia, Italy.
  • Maestri M Department of General Surgery, IRCCS Policlinico San Matteo, Pavia, Italy.
  • Cobianchi L Department of Clinical, Surgical, Diagnostic and Paediatric Sciences, University of Pavia, Pavia, Italy.
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  • 2017-11-22
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2018
Extracellular matrix
Kidney scaffold
Kidney transplantation
Organ bioengineering
Rat model
Regenerative medicine
Animals
Detergents
Extracellular Matrix
Kidney
Perfusion
Rats
Tissue Engineering
Tissue Scaffolds
English Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/46491
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