Back
The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells
Journal article

The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells

  • Holenstein, Claude N. Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Horvath, Aron Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Schär, Barbara Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Schoenenberger, Angelina D. Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Bollhalder, Maja Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Goedecke, Nils Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Bartalena, Guido Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Otto, Oliver Zentrum für Innovationskompetenz, Universität Greifswald, 17489 Greifswald, Germany
  • Herbig, Maik Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany
  • Guck, Jochen Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany
  • Müller, Daniel A. Biomechanics Laboratory, University Hospital Balgrist, University of Zürich, 8008 Zürich, Switzerland
  • Snedeker, Jess G. Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
  • Silvan, Unai Institute for Biomechanics, ETH Zurich, 8008 Zürich, Switzerland
Show more…
Published in:
  • Molecular Biology of the Cell. - American Society for Cell Biology (ASCB). - 2019, vol. 30, no. 7, p. 887-898
Cell Biology
Molecular Biology
English Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate remains below 30%. In the present work, we have used a number of analytical methods to investigate the impact of increased metastatic potential on the biophysical properties and force generation of osteosarcoma cells. With that aim, we used two paired osteosarcoma cell lines, with each one comprising a parental line with low metastatic potential and its experimentally selected, highly metastatic form. Mechanical characterization was performed by means of atomic force microscopy, tensile biaxial deformation, and real-time deformability, and cell traction was measured using two-dimensional and micropost-based traction force microscopy. Our results reveal that the low metastatic osteosarcoma cells display larger spreading sizes and generate higher forces than the experimentally selected, highly malignant variants. In turn, the outcome of cell stiffness measurements strongly depends on the method used and the state of the probed cell, indicating that only a set of phenotyping methods provides the full picture of cell mechanics.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/41655
Statistics
Document views: 15 File downloads: