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Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions.
Journal article

Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions.

  • Fetchko M Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
  • Stagljar I
  • 2004-03-09
Published in:
  • Methods (San Diego, Calif.). - 2004
DNA, Complementary
Escherichia coli
Gene Library
Genes, Reporter
Genetic Vectors
Histidine
Membrane Proteins
Models, Molecular
Mutation
Protein Binding
Protein Engineering
Recombinant Fusion Proteins
Saccharomyces cerevisiae
Transcription Factors
Transformation, Genetic
Two-Hybrid System Techniques
Ubiquitin
beta-Galactosidase
English The characterization of protein-protein interactions provides the foundation for further studies concerning protein complex function and regulation. Since the advent of the yeast two-hybrid assay, many additional genetic systems based upon the principle of protein fragment complementation have been designed. One such system, the split-ubiquitin membrane yeast two-hybrid system (MbYTH), is able to analyze the interaction status between two integral membrane proteins. This ability of the MbYTH system augments genetic analysis of protein interactions by covering for the inherent limitation of the yeast two-hybrid system when studying membrane protein interactions. Herein, we provide a description of the MbYTH method and detailed protocols in order to monitor protein interactions and discover novel interacting partners using the MbYTH system.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/4043
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