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Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis.

  • Kolb M Service of General Internal Medicine, Geneva University Hospitals (HUG), Geneva, Switzerland.
  • Lazarevic V Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.
  • Emonet S Bacteriology Laboratory, Service of Laboratory Medicine, Geneva University Hospitals, Geneva, Switzerland.
  • Calmy A Service of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.
  • Girard M Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.
  • Gaïa N Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.
  • Charretier Y Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.
  • Cherkaoui A Bacteriology Laboratory, Service of Laboratory Medicine, Geneva University Hospitals, Geneva, Switzerland.
  • Keller P Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
  • Huber C Service of Cardiovascular Surgery, Geneva University Hospitals, Geneva, Switzerland.
  • Schrenzel J Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.
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  • 2019-10-17
Published in:
  • Frontiers in medicine. - 2019
Cardiobacterium hominis
clinical metagenomics
culture-negative endocarditis
diagnosis
next-generation sequencing
English Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis.
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  • English
Open access status
gold
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Persistent URL
https://folia.unifr.ch/global/documents/38967
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