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Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions.
Journal article

Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions.

  • Picotti P Department of Biology, Institute of Biochemistry, ETH Zurich, Switzerland. paola.picotti@bc.biol.ethz.ch
  • Aebersold R
  • 2012-06-07
Published in:
  • Nature methods. - 2012
Biomarkers
Isotope Labeling
Mass Spectrometry
Metabolic Networks and Pathways
Peptide Fragments
Protein Processing, Post-Translational
Proteomics
Sensitivity and Specificity
Signal Transduction
Systems Biology
English Selected reaction monitoring (SRM) is a targeted mass spectrometry technique that is emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is particularly useful when predetermined sets of proteins, such as those constituting cellular networks or sets of candidate biomarkers, need to be measured across multiple samples in a consistent, reproducible and quantitatively precise manner. Here we describe how SRM is applied in proteomics, review recent advances, present selected applications and provide a perspective on the future of this powerful technology.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/36456
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