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A synteny-based draft genome sequence of the forage grass Lolium perenne.

  • Byrne SL Department of Molecular Biology, Genetics, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.
  • Nagy I Department of Molecular Biology, Genetics, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.
  • Pfeifer M Plant Genome and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, Neuherberg, 85764, Germany.
  • Armstead I Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, SY23 3DA, UK.
  • Swain S Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, SY23 3DA, UK.
  • Studer B Institute of Agricultural Sciences, ETH Zurich, Universitätstraße 2, 8092, Zürich, Switzerland.
  • Mayer K Plant Genome and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, Neuherberg, 85764, Germany.
  • Campbell JD Department of Molecular Biology, Genetics, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.
  • Czaban A Department of Molecular Biology, Genetics, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.
  • Hentrup S Department of Molecular Biology, Genetics, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.
  • Panitz F Department of Molecular Biology and Genetics, Research Centre Foulum, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark.
  • Bendixen C Department of Molecular Biology and Genetics, Research Centre Foulum, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark.
  • Hedegaard J Department of Molecular Biology and Genetics, Research Centre Foulum, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark.
  • Caccamo M The Genome Analysis Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
  • Asp T Department of Molecular Biology, Genetics, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark.
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  • 2015-09-27
Published in:
  • The Plant journal : for cell and molecular biology. - 2015
Lolium perenne
genome sequence
perennial ryegrass
pollen allergens
self-incompatability
Animal Feed
Flowers
Gene Expression Regulation, Plant
Gene Ontology
Genome, Plant
Lolium
Molecular Sequence Annotation
Phylogeny
Plant Breeding
Poaceae
Pollen
Pollination
Self-Incompatibility in Flowering Plants
Sequence Analysis, DNA
Synteny
Transcriptome
English Here we report the draft genome sequence of perennial ryegrass (Lolium perenne), an economically important forage and turf grass species that is widely cultivated in temperate regions worldwide. It is classified along with wheat, barley, oats and Brachypodium distachyon in the Pooideae sub-family of the grass family (Poaceae). Transcriptome data was used to identify 28,455 gene models, and we utilized macro-co-linearity between perennial ryegrass and barley, and synteny within the grass family, to establish a synteny-based linear gene order. The gametophytic self-incompatibility mechanism enables the pistil of a plant to reject self-pollen and therefore promote out-crossing. We have used the sequence assembly to characterize transcriptional changes in the stigma during pollination with both compatible and incompatible pollen. Characterization of the pollen transcriptome identified homologs to pollen allergens from a range of species, many of which were expressed to very high levels in mature pollen grains, and are potentially involved in the self-incompatibility mechanism. The genome sequence provides a valuable resource for future breeding efforts based on genomic prediction, and will accelerate the development of new varieties for more productive grasslands.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/31201
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