Calcium Uncaging with Visible Light.
- Agarwal HK Department of Neuroscience, Mount Sinai School of Medicine , New York, New York 10029, United States.
- Janicek R Department of Physiology, University of Bern , Bern CH 3012, Switzerland.
- Chi SH School of Chemistry and Biochemistry, Center for Organic Photonics and Electronics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
- Perry JW School of Chemistry and Biochemistry, Center for Organic Photonics and Electronics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
- Niggli E Department of Physiology, University of Bern , Bern CH 3012, Switzerland.
- Ellis-Davies GC Department of Neuroscience, Mount Sinai School of Medicine , New York, New York 10029, United States.
- 2016-03-15
Published in:
- Journal of the American Chemical Society. - 2016
English
We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε440 = 66,000 M(-1) cm(-1) and two-photon cross section of 350 GM at 775 nm). We developed a "caged calcium" molecule by conjugation of BIST to a Ca(2+) chelator that upon laser flash photolysis rapidly releases Ca(2+) in <0.2 ms. Using the patch-clamp method the optical probe, loaded with Ca(2+), was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca(2+) induced highly local or cell-wide physiological Ca(2+) signaling events.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1021/jacs.5b11606
- PMID 26974387
- Persistent URL
- https://folia.unifr.ch/global/documents/285748
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