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The Cre/Lox System to Assess the Development of the Mouse Brain.
Journal article

The Cre/Lox System to Assess the Development of the Mouse Brain.

  • Kratochwil CF Zoology and Evolutionary Biology, Department of Biology, University of Konstanz, Konstanz, Germany.
  • Rijli FM Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland. Filippo.Rijli@fmi.ch.
  • 2019-09-26
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2020
Brainbow
CRISPR-Cas9
Conditional knockin
Conditional knockout
Cre recombinase
CreERT2
Flpe recombinase
MADM
Split-Cre
Transgenesis
Animals
Brain
Clustered Regularly Interspaced Short Palindromic Repeats
DNA Nucleotidyltransferases
Female
Genotyping Techniques
Integrases
Mice
Pregnancy
Recombination, Genetic
English Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knockouts) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality or pleiotropy can be circumvented. Furthermore, Cre-mediated recombination has broad applications in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter gives an overview about applications for the Cre/LoxP system and their execution.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/282924
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