Back
Full-text
Journal article

Calcium Polyphosphate Nanoparticles Act as an Effective Inorganic Phosphate Source during Osteogenic Differentiation of Human Mesenchymal Stem Cells.

  • Phelipe Hatt L AO Research Institute Davos, 7270 Davos Platz, Switzerland.
  • Thompson K AO Research Institute Davos, 7270 Davos Platz, Switzerland.
  • Müller WEG Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, Germany.
  • Stoddart MJ AO Research Institute Davos, 7270 Davos Platz, Switzerland.
  • Armiento AR AO Research Institute Davos, 7270 Davos Platz, Switzerland.
  • 2019-11-23
Published in:
  • International journal of molecular sciences. - 2019
Ca-polyphosphate nanoparticles
inorganic polyphosphate
mesenchymal stem cells
osteogenic differentiation
β-glycerolphosphate
Alkaline Phosphatase
Calcium
Calcium Phosphates
Cell Culture Techniques
Cell Differentiation
Cells, Cultured
Gene Expression Regulation
Glycerophosphates
Humans
Mesenchymal Stem Cells
Nanoparticles
Osteogenesis
Polyphosphates
English The ability of bone-marrow-derived mesenchymal stem/stromal cells (BM-MSCs) to differentiate into osteoblasts makes them the ideal candidate for cell-based therapies targeting bone-diseases. Polyphosphate (polyP) is increasingly being studied as a potential inorganic source of phosphate for extracellular matrix mineralisation. The aim of this study is to investigate whether polyP can effectively be used as a phosphate source during the in vitro osteogenic differentiation of human BM-MSCs. Human BM-MSCs are cultivated under osteogenic conditions for 28 days with phosphate provided in the form of organic β-glycerolphosphate (BGP) or calcium-polyP nanoparticles (polyP-NP). Mineralisation is demonstrated using Alizarin red staining, cellular ATP content, and free phosphate levels are measured in both the cells and the medium. The effects of BGP or polyP-NP on alkaline phosphatase (ALP) activity and gene expression of a range of osteogenic-related markers are also assessed. PolyP-NP supplementation displays comparable effects to the classical BGP-containing osteogenic media in terms of mineralisation, ALP activity and expression of osteogenesis-associated genes. This study shows that polyP-NP act as an effective source of phosphate during mineralisation of BM-MSC. These results open new possibilities with BM-MSC-based approaches for bone repair to be achieved through doping of conventional biomaterials with polyP-NP.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/273371
Statistics
Document views: 47 File downloads:
  • Full-text: 0