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A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects.
Journal article

A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects.

  • Pfister A Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Barberon M Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Alassimone J Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Kalmbach L Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Lee Y Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Vermeer JE Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Yamazaki M Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Li G Biochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, France.
  • Maurel C Biochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, France.
  • Takano J Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan.
  • Kamiya T Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United Kingdom.
  • Salt DE Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United Kingdom.
  • Roppolo D Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
  • Geldner N Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
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  • 2014-09-19
Published in:
  • eLife. - 2014
arabidopsis
cell biology
plant biology
plant nutrition
polarity
root biology
Arabidopsis
Arabidopsis Proteins
Biological Transport
COP9 Signalosome Complex
Cell Differentiation
Diffusion
Gene Expression Profiling
Gene Expression Regulation, Plant
Homeostasis
Lipids
Microscopy, Confocal
Mutation
Nuclear Proteins
Oligonucleotide Array Sequence Analysis
Plant Roots
Plants, Genetically Modified
Potassium
Protein Kinases
Reverse Transcriptase Polymerase Chain Reaction
Water
English The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)--major players of endodermal differentiation--into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/27275
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