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Structural Transformation in Vesicles upon Hydrolysis of Phosphatidylethanolamine and Phosphatidylcholine with Phospholipase C.

  • Fong WK Department of Health Sciences & Technology , ETH Zürich , 8092 Zürich , Switzerland.
  • Sánchez-Ferrer A Department of Health Sciences & Technology , ETH Zürich , 8092 Zürich , Switzerland.
  • Rappolt M School of Food Science and Nutrition , University of Leeds , LS2 9JT Leeds , Yorkshire , U.K.
  • Boyd BJ Drug Delivery, Disposition and Dynamics, and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences , Monash University , Parkville Campus, 381 Royal Parade , Parkville , 3052 Victoria , Australia.
  • Mezzenga R Department of Health Sciences & Technology , ETH Zürich , 8092 Zürich , Switzerland.
  • 2019-10-24
Published in:
  • Langmuir : the ACS journal of surfaces and colloids. - 2019
Drug Delivery Systems
Drug Liberation
Hydrolysis
Lipid Bilayers
Magnetic Resonance Spectroscopy
Membrane Lipids
Micelles
Microscopy, Electron, Scanning
Phosphatidylcholines
Phosphatidylethanolamines
Phospholipids
Scattering, Small Angle
Type C Phospholipases
X-Ray Diffraction
English This study provides insights into dynamic nanostructural changes in phospholipid systems during hydrolysis with phospholipase C, the fate of the hydrolysis products, and the kinetics of lipolysis. The effect of lipid restructuring of the vesicle was investigated using small-angle X-ray scattering and cryogenic scanning electron microscopy. The rate and extent of phospholipid hydrolysis were quantified using nuclear magnetic resonance. Hydrolysis of two phospholipids, phosphatidylethanolamine (PE) and phosphatidylcholine (PC), results in the cleavage of the molecular headgroup, causing two strikingly different changes in lipid self-assembly. The diacylglycerol product of PC escapes the lipid bilayer, whereas the diacylglycerol product adopts a different configuration within the lipid bilayer of the PE vesicles. These results are then discussed concerning the change of the lipid configuration upon the lipid membrane and its potential implications in vivo, which is of significant importance for the detailed understanding of the fate of lipidic particles and the rational design of enzyme-responsive lipid-based drug delivery systems.
Language
  • English
Open access status
green
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Persistent URL
https://folia.unifr.ch/global/documents/272433
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