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Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses.
Journal article

Quantification of THC in Cannabis plants by fast-HPLC-DAD: A promising method for routine analyses.

  • Burnier C School of Criminal Justice, University of Lausanne, Switzerland.
  • Esseiva P School of Criminal Justice, University of Lausanne, Switzerland.
  • Roussel C Section of Chemistry and Chemical Engineering, Ecole Polytechnique Fédérale de Lausanne, Switzerland. Electronic address: christophe.roussel@epfl.ch.
  • 2018-10-24
Published in:
  • Talanta. - 2019
Cannabis
Fast-HPLC-DAD
THC-A
Δ(9)-THC
Calibration
Cannabis
Chromatography, High Pressure Liquid
Dronabinol
Gas Chromatography-Mass Spectrometry
Plant Extracts
English Nowadays, Gas Chromatography Mass Spectrometry (GC-MS) is mainly used in forensic sciences but suffers from limitations when the analysed compounds are thermally instable as it is the case for THC-A (Tetrahydrocannabinolic Acid) which is converted into Δ9-THC (Δ9-Tetrahydrocannabinol) that subsequently partially degrades. We propose herein a Fast High Pressure Liquid Chromatography (Fast-HPLC-DAD) method which allows the efficient separation of CBN (Cannabinol), CBD (Cannabidiol), THC-A and Δ9-THC, the major cannabinoids compounds found in cannabis plants in less than 5 min. Our method allows also the proper quantification of Δ9-THC in plant extracts using an external calibration method with a very good accuracy as pointed out by a recovery of 100.53 ± 3.12%. It is also an interesting low cost alternative to Ultra High Pressure Liquid Chromatography (UPLC) for routine analyses in forensic sciences.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/259527
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