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Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix®.
Journal article

Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix®.

  • Martoccia C Swiss Federal Institute of Technology (EPFL), Institute of Chemical Sciences and Engineering, Station 6, CH-1015 Lausanne, Switzerland.
  • Zellweger M Swiss Federal Institute of Technology (EPFL), Institute of Chemical Sciences and Engineering, Station 6, CH-1015 Lausanne, Switzerland.
  • Lovisa B Swiss Federal Institute of Technology (EPFL), Institute of Chemical Sciences and Engineering, Station 6, CH-1015 Lausanne, Switzerland.
  • Jichlinski P University Hospital (CHUV), Department of Urology, BH-10, Rue du Bugnon 46, CH-1011 Lausanne, Switzerland.
  • van den Bergh H Swiss Federal Institute of Technology (EPFL), Institute of Chemical Sciences and Engineering, Station 6, CH-1015 Lausanne, Switzerland.
  • Wagnières G Swiss Federal Institute of Technology (EPFL), Institute of Chemical Sciences and Engineering, Station 6, CH-1015 Lausanne, Switzerland.
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  • 2014-09-11
Published in:
  • Journal of biomedical optics. - 2014
Adult
Aged
Aminolevulinic Acid
Cystoscopy
Female
Fluorescence
Humans
Hydrogen-Ion Concentration
Male
Middle Aged
Spectrometry, Fluorescence
Temperature
Urinary Bladder
Urine
English Fluorescence cystoscopy enhances detection of early bladder cancer. Water used to inflate thebladder during the procedure rapidly contains urine, which may contain fluorochromes. This frequently degradesfluorescence images. Samples of bladder washout fluid (BWF) or urine were collected (15 subjects). We studiedtheir fluorescence properties and assessed changes induced by pH (4 to 9) and temperature (15°C to 41°C).A typical fluorescence spectrum of BWF features a main peak (excitation/emission: 320∕420 nm, FWHM =50∕100 nm) and a weaker (5% to 20% of main peak intensity), secondary peak (excitation/emission: 455∕525 nm, FWHM = 80∕50 nm). Interpatient fluctuations of fluorescence intensity are observed. Fluorescence intensity decreases when temperature increases (max 30%) or pH values vary (max 25%). Neither approach is compatible with clinical settings. Fluorescence lifetime measurements suggest that 4-pyridoxic acid/riboflavin is the most likely molecule responsible for urine’s main/secondary fluorescence peak. Our measurements give an insight into the spectroscopy of the detrimental background fluorescence. This should be included in the optical design of fluorescence cystoscopes. We estimate that restricting the excitation range from 370–430 nm to 395–415 nm would reduce the BWF background by a factor 2.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/259293
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