The forensiX Evidence Collection Tube and Its Impact on DNA Preservation and Recovery
- Garvin, Alex M. Confarma France SARL, Zone Industrielle Canal d’Alsace, 68490 Hombourg, France
- Holzinger, Ralf ORCID Confarma France SARL, Zone Industrielle Canal d’Alsace, 68490 Hombourg, France
- Berner, Florian ORCID Institute of Chemistry and Biological Chemistry, Zurich University of Applied Sciences (ZHAW), Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland
- Krebs, Walter ORCID Institute of Chemistry and Biological Chemistry, Zurich University of Applied Sciences (ZHAW), Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland
- Hostettler, Bernhard ORCID Prionics AG, Wagistrasse 27a, 8952 Schlieren, Switzerland
- Lardi, Elges ORCID Prionics AG, Wagistrasse 27a, 8952 Schlieren, Switzerland
- Hertli, Christian ORCID Prionics AG, Wagistrasse 27a, 8952 Schlieren, Switzerland
- Quartermaine, Roy ORCID Prionics AG, Wagistrasse 27a, 8952 Schlieren, Switzerland
- Stamm, Christoph ORCID Prionics AG, Wagistrasse 27a, 8952 Schlieren, Switzerland
Published in:
- BioMed Research International. - Hindawi Limited. - 2013, vol. 2013, p. 1-7
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Medicine
English
Biological samples are vulnerable to degradation from the time they are collected until they are analysed at the laboratory. Biological contaminants, such as bacteria, fungi, and enzymes, as well as environmental factors, such as sunlight, heat, and humidity, can increase the rate of DNA degradation. Currently, DNA samples are normally dried or frozen to limit their degradation prior to their arrival at the laboratory. In this study, the effect of the sample drying rate on DNA preservation was investigated, as well as a comparison between drying and freezing methods. The drying performances of two commercially available DNA collection tools (swab and drying tube) with different drying rates were evaluated. The swabs were used to collect human saliva, placed into the drying tubes, and stored in a controlled environment at 25°C and 60% relative humidity, or frozen at −20°C, for 2 weeks. Swabs that were stored in fast sample drying tubes yielded 95% recoverable DNA, whereas swabs stored in tubes with slower sample drying rates yielded only 12% recoverable DNA; saliva stored in a microtube at −20°C was used as a control. Thus, DNA sampling tools that offer rapid drying can significantly improve the preservation of DNA collected on a swab, increasing the quantity of DNA available for subsequent analysis.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1155/2013/105797
- ISSN 2314-6133
- Persistent URL
- https://folia.unifr.ch/global/documents/256989
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