Back
Full-text
Journal article

Megahertz serial crystallography.

  • Wiedorn MO Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Oberthür D Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Bean R European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Schubert R The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Werner N Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, Universität Hamburg, Notkestrasse 85, 22607, Hamburg, Germany.
  • Abbey B Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, 3086, Australia.
  • Aepfelbacher M Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), 20246, Hamburg, Germany.
  • Adriano L Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Allahgholi A Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Al-Qudami N European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Andreasson J Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, 751 24, Sweden.
  • Aplin S Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Awel S Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Ayyer K Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Bajt S Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Barák I Institute of Molecular Biology, SAS, Dubravska cesta 21, 845 51, Bratislava, Slovakia.
  • Bari S Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Bielecki J European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Botha S The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Boukhelef D European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Brehm W Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Brockhauser S European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Cheviakov I Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), 20246, Hamburg, Germany.
  • Coleman MA Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
  • Cruz-Mazo F Depart. Ingeniería Aeroespacial y Mecánica de Fluidos ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.
  • Danilevski C European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Darmanin C Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, 3086, Australia.
  • Doak RB Max Planck Institute for Medical Research, Jahnstr. 29, 69120, Heidelberg, Germany.
  • Domaracky M Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Dörner K European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Du Y Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Fangohr H European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Fleckenstein H Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Frank M Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
  • Fromme P School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Gañán-Calvo AM Depart. Ingeniería Aeroespacial y Mecánica de Fluidos ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.
  • Gevorkov Y Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Giewekemeyer K European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Ginn HM Division of Structural Biology, Headington, Oxford, OX3 7BN, UK.
  • Graafsma H Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Graceffa R European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Greiffenberg D Paul Scherrer Institut, Forschungsstrasse 111, 5232, Villigen, Switzerland.
  • Gumprecht L Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Göttlicher P Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Hajdu J Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, 751 24, Sweden.
  • Hauf S European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Heymann M Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 82152, Martinsried, Germany.
  • Holmes S Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, 3086, Australia.
  • Horke DA Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Hunter MS Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, 94025, CA, USA.
  • Imlau S Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Kaukher A European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Kim Y European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Klyuev A Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Knoška J Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Kobe B School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, QLD, 4072, Australia.
  • Kuhn M Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Kupitz C Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, WI, 53211, USA.
  • Küpper J Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Lahey-Rudolph JM Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Laurus T Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Le Cong K Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, Universität Hamburg, Notkestrasse 85, 22607, Hamburg, Germany.
  • Letrun R European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Xavier PL Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Maia L European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Maia FRNC Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, 751 24, Sweden.
  • Mariani V Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Messerschmidt M European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Metz M Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Mezza D Paul Scherrer Institut, Forschungsstrasse 111, 5232, Villigen, Switzerland.
  • Michelat T European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Mills G European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Monteiro DCF The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Morgan A Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Mühlig K Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, 751 24, Sweden.
  • Munke A Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, 751 24, Sweden.
  • Münnich A European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Nette J The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Nugent KA Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, 3086, Australia.
  • Nuguid T Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, Universität Hamburg, Notkestrasse 85, 22607, Hamburg, Germany.
  • Orville AM Diamond Light Source, Research Complex at Harwell, and University of Oxford, Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, UK.
  • Pandey S Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, WI, 53211, USA.
  • Pena G Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Villanueva-Perez P Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Poehlsen J Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Previtali G European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Redecke L Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), 20246, Hamburg, Germany.
  • Riekehr WM Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany.
  • Rohde H Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), 20246, Hamburg, Germany.
  • Round A European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Safenreiter T Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Sarrou I Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Sato T Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Schmidt M Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, WI, 53211, USA.
  • Schmitt B Paul Scherrer Institut, Forschungsstrasse 111, 5232, Villigen, Switzerland.
  • Schönherr R Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany.
  • Schulz J European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Sellberg JA Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, Stockholm, 106 91, Sweden.
  • Seibert MM Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, 751 24, Sweden.
  • Seuring C Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Shelby ML Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
  • Shoeman RL Max Planck Institute for Medical Research, Jahnstr. 29, 69120, Heidelberg, Germany.
  • Sikorski M European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Silenzi A European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Stan CA Physics Department, Rutgers University Newark, Newark, NJ, 07102, USA.
  • Shi X Paul Scherrer Institut, Forschungsstrasse 111, 5232, Villigen, Switzerland.
  • Stern S Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Sztuk-Dambietz J European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Szuba J European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Tolstikova A Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Trebbin M The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Trunk U Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Vagovic P Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Ve T Institute for Glycomics, Griffith University, Southport, QLD, 4222, Australia.
  • Weinhausen B European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • White TA Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Wrona K European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Xu C European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Yefanov O Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany.
  • Zatsepin N Department of Physics, Arizona State University, Tempe, AZ, 85287, USA.
  • Zhang J Paul Scherrer Institut, Forschungsstrasse 111, 5232, Villigen, Switzerland.
  • Perbandt M The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Mancuso AP European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Betzel C The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Chapman H Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany. henry.chapman@desy.de.
  • Barty A Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany. anton.barty@desy.de.
Show more…
  • 2018-10-04
Published in:
  • Nature communications. - 2018
English The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/25312
Statistics
Document views: 46 File downloads:
  • Full-text: 0