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Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions

  • Patrikoski, Mimmi ORCID Science Center, Tampere University Hospital, Tampere, Finland
  • Lee, Michelle Hui Ching Department of Biomedical Engineering, National University of Singapore, Singapore 117575
  • Mäkinen, Laura Science Center, Tampere University Hospital, Tampere, Finland
  • Ang, Xiu Min Department of Biomedical Engineering, National University of Singapore, Singapore 117575
  • Mannerström, Bettina Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
  • Raghunath, Michael Institute of Chemistry and Biology, Zurich University of Applied Sciences (ZHAW), 8820 Wädenswil, Switzerland
  • Miettinen, Susanna Science Center, Tampere University Hospital, Tampere, Finland
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  • Stem Cells International. - Hindawi Limited. - 2017, vol. 2017, p. 1-14
English Microenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media. Furthermore, the immunophenotype of ASCs under MMC was evaluated. The proliferative capacity of ASCs under MMC was attenuated in each condition. However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures. Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures. In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC. The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54. However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions. Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent. In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation.
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  • English
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gold
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https://folia.unifr.ch/global/documents/249214
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