Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut.
- Bastiaan-Net S Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.
- Pina-Pérez MC Institute of Life Technologies, HES-SO Valais-Wallis, Sion, Switzerland.
- Dekkers BJW Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands.
- Westphal AH Biochemistry, Wageningen University and Research, Wageningen, The Netherlands.
- America AHP Wageningen Plant Research, Wageningen University and Research, Wageningen, The Netherlands.
- Ariëns RMC Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.
- de Jong NW Allergology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands.
- Wichers HJ Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.
- Mes JJ Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.
- 2020-03-29
Published in:
- Protein science : a publication of the Protein Society. - 2020
Anacardium occidentale
Bet v 1-like
PR10
RNA-seq
cashew nut
in silico allergenicity analysis
oral allergy syndrome (OAS)
English
Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1002/pro.3856
- PMID 32219913
- Persistent URL
- https://folia.unifr.ch/global/documents/243259
Statistics
Document views: 17
File downloads:
- fulltext.pdf: 0