Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.
- Mitterer V Institute for Molecular Biosciences, University of Graz, Humboldtstrasse 50, 8010, Graz, Austria.
- Shayan R Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 118 route de Narbonne, 31062, Toulouse Cedex, France.
- Ferreira-Cerca S Biochemistry III - Institute for Biochemistry, Genetics and Microbiology, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany. sebastien.ferreira-cerca@ur.de.
- Murat G Unit of Biochemistry, Department of Biology, University of Fribourg, Chemin du Musée 10, 1700, Fribourg, Switzerland.
- Enne T Institute for Molecular Biosciences, University of Graz, Humboldtstrasse 50, 8010, Graz, Austria.
- Rinaldi D Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 118 route de Narbonne, 31062, Toulouse Cedex, France.
- Weigl S Institute for Molecular Biosciences, University of Graz, Humboldtstrasse 50, 8010, Graz, Austria.
- Omanic H Institute for Molecular Biosciences, University of Graz, Humboldtstrasse 50, 8010, Graz, Austria.
- Gleizes PE Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 118 route de Narbonne, 31062, Toulouse Cedex, France.
- Kressler D Unit of Biochemistry, Department of Biology, University of Fribourg, Chemin du Musée 10, 1700, Fribourg, Switzerland. dieter.kressler@unifr.ch.
- Plisson-Chastang C Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 118 route de Narbonne, 31062, Toulouse Cedex, France. plisson@ibcg.biotoul.fr.
- Pertschy B Institute for Molecular Biosciences, University of Graz, Humboldtstrasse 50, 8010, Graz, Austria. brigitte.pertschy@uni-graz.at.
- 2019-06-23
Published in:
- Nature communications. - 2019
English
Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41467-019-10678-z
- PMID 31227701
- Persistent URL
- https://folia.unifr.ch/global/documents/242385
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