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Specific Targeting of Proerythroblasts and Erythroleukemic Cells by the VP1u Region of Parvovirus B19.
Journal article

Specific Targeting of Proerythroblasts and Erythroleukemic Cells by the VP1u Region of Parvovirus B19.

  • Leisi R Department of Chemistry and Biochemistry, University of Bern , 3012 Bern, Switzerland.
  • von Nordheim M Department of Chemistry and Biochemistry, University of Bern , 3012 Bern, Switzerland.
  • Kempf C Department of Chemistry and Biochemistry, University of Bern , 3012 Bern, Switzerland.
  • Ros C Department of Chemistry and Biochemistry, University of Bern , 3012 Bern, Switzerland.
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  • 2015-08-05
Published in:
  • Bioconjugate chemistry. - 2015
Avidin
Biological Transport
Capsid Proteins
Cell Line, Tumor
Drug Carriers
Erythroblasts
Humans
Leukemia, Erythroblastic, Acute
Models, Molecular
Oligonucleotides
Parvovirus B19, Human
Protein Conformation
English Viruses are evolutionarily developed cell-entering nanomachines, which are frequently used as gene or drug delivery systems. Parvovirus B19 (B19V) shows a remarkably restricted tropism for erythropoietin-dependent erythroid differentiation stages, and thus this virus provides an opportunity to deliver cargo to these intermediate differentiated cells. Here we report the construction of a delivery system from B19V subunits that maintains the highly selective cell-entry of the native virus and offers versatile cargo transport. To obtain this specific carrier, we conjugated the cell-targeting VP1u region of B19V to NeutrAvidin as a loading platform for biotinylated cargos. The VP1u-NeutrAvidin conjugate delivered fluorophores, DNA, and toxic payloads specifically to erythroid cells around the proerythroblast differentiation stage, including erythroleukemic cells. The VP1u-NeutrAvidin represents a unique cell surface marker which exclusively detects intermediate erythroid differentiation stages. Furthermore, the cell-entering property of this viral-based targeting system offers opportunities for erythroid-specific drug delivery or gene therapy.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/234799
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