Journal article
Regeneration patterns influence hindlimb automutilation after sciatic nerve repair using stem cells in rats.
- Haselbach D Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Lausanne (CHUV), Rue du Bugnon 46, 1011 Lausanne, Switzerland. Electronic address: Daniel.Haselbach@chuv.ch.
- Raffoul W Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Lausanne (CHUV), Rue du Bugnon 46, 1011 Lausanne, Switzerland. Electronic address: Wassim.Raffoul@chuv.ch.
- Larcher L Department of Plastic, Reconstructive and Hand Surgery, Brixen Hospital, South Tyrolean Health Care Service (SABES), Dantersrasse 51-I-3942, Brixen, Italy. Electronic address: lorenz.larcher@gmail.com.
- Tremp M Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Basel, Spitalstrasse 21, CH-4031 Basel, Switzerland. Electronic address: Mathias.Tremp@usb.ch.
- Kalbermatten DF Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Basel, Spitalstrasse 21, CH-4031 Basel, Switzerland. Electronic address: Daniel.Kalbermatten@usb.ch.
- di Summa PG Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Lausanne (CHUV), Rue du Bugnon 46, 1011 Lausanne, Switzerland. Electronic address: Pietro.di-Summa@chuv.ch.
- 2016-11-07
Published in:
- Neuroscience letters. - 2016
Autophagy
Neuropathic pain
Regenerative stem cells
Sciatic
Adipose Tissue
Adult Stem Cells
Animals
Autophagy
Fibrin
Hindlimb
Male
Mesenchymal Stem Cells
Nerve Regeneration
Neuralgia
Rats, Sprague-Dawley
Schwann Cells
Sciatic Nerve
Self Mutilation
Tissue Scaffolds
English
INTRODUCTION
Hindlimb autophagy is common after rat sciatic total axotomy and is considered as a sign of neuropathic pain. We applied adult stem cells in a fibrin conduit in a total sciatic axotomy model to improve nerve regeneration, investigating whether a correlation could be detected between stem cells effects on regeneration and limb autophagy.
MATERIAL AND METHODS
After sciatic nerve section, a 1-cm sciatic gap was crossed using fibrin conduits. Experimental groups included empty fibrin conduits, fibrin conduits seeded with primary Schwann cells, and fibrin conduits seeded with Schwann cell-like differentiated mesenchymal or adipose-derived stem cells (dMSCs and dASCs). Controls were represented by autografts and by sham rats (tot n=34). At 16 weeks post-implantation, regeneration pattern was analysed on histological sections and related to eventual autophagy. Hindlimbs were evaluated and scored according to autophagy Wall's scale and X-Rays radiological evaluation.
RESULTS
All regenerative cell lines significantly improved myelination at the mid conduit level, compared to the empty tubes. However, dMSC could not significantly improve myelination at the distal stump, showing a more chaotic regeneration compared to both other cells groups and controls. Autophagy was correlated to this regeneration patterns, with higher autophagy scores in the empty and dMSC group.
CONCLUSION
Hindlimb autophagy can be used as index of neuropathic pain due to nerve lesion or on-going immature regeneration. dMSC group was characterized by a less targeted regeneration comparing to dASC and primary Schwann cells, which confirmed their effectiveness in regeneration and potential in future clinical applications.
Hindlimb autophagy is common after rat sciatic total axotomy and is considered as a sign of neuropathic pain. We applied adult stem cells in a fibrin conduit in a total sciatic axotomy model to improve nerve regeneration, investigating whether a correlation could be detected between stem cells effects on regeneration and limb autophagy.
MATERIAL AND METHODS
After sciatic nerve section, a 1-cm sciatic gap was crossed using fibrin conduits. Experimental groups included empty fibrin conduits, fibrin conduits seeded with primary Schwann cells, and fibrin conduits seeded with Schwann cell-like differentiated mesenchymal or adipose-derived stem cells (dMSCs and dASCs). Controls were represented by autografts and by sham rats (tot n=34). At 16 weeks post-implantation, regeneration pattern was analysed on histological sections and related to eventual autophagy. Hindlimbs were evaluated and scored according to autophagy Wall's scale and X-Rays radiological evaluation.
RESULTS
All regenerative cell lines significantly improved myelination at the mid conduit level, compared to the empty tubes. However, dMSC could not significantly improve myelination at the distal stump, showing a more chaotic regeneration compared to both other cells groups and controls. Autophagy was correlated to this regeneration patterns, with higher autophagy scores in the empty and dMSC group.
CONCLUSION
Hindlimb autophagy can be used as index of neuropathic pain due to nerve lesion or on-going immature regeneration. dMSC group was characterized by a less targeted regeneration comparing to dASC and primary Schwann cells, which confirmed their effectiveness in regeneration and potential in future clinical applications.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.neulet.2016.10.024
- PMID 27760382
- Persistent URL
- https://folia.unifr.ch/global/documents/232289
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