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Journal article

Quantification of the antiplasmodial alkaloid carpaine in papaya (Carica papaya) leaves.

  • Julianti T Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
  • Oufir M Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
  • Hamburger M Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
  • 2014-08-26
Published in:
  • Planta medica. - 2014
Alkaloids
Antimalarials
Carica
Chromatography, High Pressure Liquid
Plant Extracts
Plant Leaves
Tandem Mass Spectrometry
English Daily consumption of papaya (Carica papaya) leaves as greens and an herbal infusion is common in some parts of Indonesia as a means for preventing malaria. Antiplasmodial activity of the leaf extracts and of the main alkaloid carpaine were recently confirmed. A quantitative assay for determination of carpaine in papaya leaves was developed and validated. The assay involved pressurized liquid extraction and quantification with the aid of ultrahigh-performance liquid chromatography-tandem mass spectroscopy. Extraction conditions were optimized with respect to solvent, temperature, and number of extraction cycles. The ultrahigh-performance liquid chromatography-tandem mass spectroscopy assay was validated over a range of 20-5000 ng/mL (R(2) of 0.9908). A total of 29 papaya leaf samples were analyzed, and carpaine concentration in dry leaves was found to range from 0.02 to 0.31%. No obvious dependence on geographic origin and leaf maturity was observed.
Language
  • English
Open access status
green
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https://folia.unifr.ch/global/documents/231978
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