Optimization of 3D-Visualization of Micro-Anatomical Structures of the Human Inner Ear in Osmium Tetroxide Contrast Enhanced Micro-CT Scans.
- van den Boogert T Division of Balance Disorders, Department of Otorhinolaryngology, Head, and Neck Surgery, Faculty of Health Medicine and Life Sciences, School for Mental Health and Neuroscience, Maastricht University Medical Centre, Maastricht, Netherlands.
- van Hoof M Division of Balance Disorders, Department of Otorhinolaryngology, Head, and Neck Surgery, Faculty of Health Medicine and Life Sciences, School for Mental Health and Neuroscience, Maastricht University Medical Centre, Maastricht, Netherlands.
- Handschuh S VetCore Facility for Research, University of Veterinary Medicine Vienna, Vienna, Austria.
- Glueckert R Department of Otorhinolaryngology, Medical University Innsbruck, Innsbruck, Austria.
- Guinand N Department of Clinical Neurosciences, Service of Otorhinolaryngology, Head, and Neck Surgery, Geneva University Hospitals, Geneva, Switzerland.
- Guyot JP Department of Clinical Neurosciences, Service of Otorhinolaryngology, Head, and Neck Surgery, Geneva University Hospitals, Geneva, Switzerland.
- Kingma H Division of Balance Disorders, Department of Otorhinolaryngology, Head, and Neck Surgery, Faculty of Health Medicine and Life Sciences, School for Mental Health and Neuroscience, Maastricht University Medical Centre, Maastricht, Netherlands.
- Perez-Fornos A Department of Clinical Neurosciences, Service of Otorhinolaryngology, Head, and Neck Surgery, Geneva University Hospitals, Geneva, Switzerland.
- Seppen B Division of Balance Disorders, Department of Otorhinolaryngology, Head, and Neck Surgery, Faculty of Health Medicine and Life Sciences, School for Mental Health and Neuroscience, Maastricht University Medical Centre, Maastricht, Netherlands.
- Johnson Chacko L Department of Otorhinolaryngology, Medical University Innsbruck, Innsbruck, Austria.
- Schrott-Fischer A Department of Otorhinolaryngology, Medical University Innsbruck, Innsbruck, Austria.
- van de Berg R Division of Balance Disorders, Department of Otorhinolaryngology, Head, and Neck Surgery, Faculty of Health Medicine and Life Sciences, School for Mental Health and Neuroscience, Maastricht University Medical Centre, Maastricht, Netherlands.
- 2018-06-07
Published in:
- Frontiers in neuroanatomy. - 2018
3D rendering
Micro-CT
cochlear implants
contrast enhancement staining
image processing
inner ear
temporal bones
vestibular implants
English
Introduction: Knowledge of the neuro-anatomical architecture of the inner ear contributes to the improvement and development of cochlear and vestibular implants. The present knowledge is mainly based on two-dimensional images (histology) or derived models that simplify the complexity of this architecture. This study investigated the feasibility of visualizing relevant neuro-anatomical structures of the inner ear in a dynamic three-dimensional reproduction, using a combination of staining, micro-CT imaging and an image processing algorithm. Methods: Four fresh cadaveric temporal bones were postfixed with osmium tetroxide (OsO4) and decalcified with EDTA. Micro-CT was used for scanning at 10 μm (4 scans) and 5.5 μm (1 scan) voxel resolution. A new image processing algorithm was developed and the scans were visualized in open source software. Results: OsO4 enhanced the contrast in all scans and the visualization was substantially improved by the image processing algorithm. The three-dimensional renderings provided detailed visualization of the whole inner ear. Details were visible up to the size of individual neurons, nerve crossings and the specific neuro-anatomical structures such as the tunnel of Corti. Conclusion: The combination of OsO4, micro-CT and the proposed image processing algorithm provides an accurate and detailed visualization of the three-dimensional micro-anatomy of the human inner ear.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.3389/fnana.2018.00041
- PMID 29872380
- Persistent URL
- https://folia.unifr.ch/global/documents/229970
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