The CAFA challenge reports improved protein function prediction and new functional annotations for hundreds of genes through experimental screens.
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Zhou N
Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA.
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Jiang Y
Indiana University Bloomington, Bloomington, Indiana, USA.
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Bergquist TR
Department of Biomedical Informatics and Medical Education, University of Washington, Seattle, WA, USA.
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Lee AJ
Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA, USA.
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Kacsoh BZ
Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
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Crocker AW
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
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Lewis KA
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
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Georghiou G
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, United Kingdom.
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Nguyen HN
Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA.
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Hamid MN
Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA.
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Davis L
Program in Bioinformatics and Computational Biology, Ames, IA, USA.
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Dogan T
Department of Computer Engineering, Hacettepe University, Ankara, Turkey.
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Atalay V
Department of Computer Engineering, Middle East Technical University (METU), Ankara, Turkey.
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Rifaioglu AS
Department of Computer Engineering, Middle East Technical University (METU), Ankara, Turkey.
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Dalkıran A
Department of Computer Engineering, Middle East Technical University (METU), Ankara, Turkey.
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Cetin Atalay R
CanSyL, Graduate School of Informatics, Middle East Technical University, Ankara, Turkey.
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Zhang C
Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
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Hurto RL
Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.
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Freddolino PL
Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
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Zhang Y
Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
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Bhat P
Achira Labs, Bangalore, India.
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Supek F
Institute for Research in Biomedicine (IRB Barcelona), Barcelona, Spain.
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Fernández JM
INB Coordination Unit, Life Sciences Department, Barcelona Supercomputing Center, Barcelona, Catalonia, Spain.
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Gemovic B
Laboratory for Bioinformatics and Computational Chemistry, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
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Perovic VR
Laboratory for Bioinformatics and Computational Chemistry, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
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Davidović RS
Laboratory for Bioinformatics and Computational Chemistry, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
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Sumonja N
Laboratory for Bioinformatics and Computational Chemistry, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
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Veljkovic N
Laboratory for Bioinformatics and Computational Chemistry, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
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Asgari E
Molecular Cell Biomechanics Laboratory, Departments of Bioengineering, University of California Berkeley, Berkeley, CA, USA.
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Mofrad MRK
Departments of Bioengineering and Mechanical Engineering, Berkeley, CA, USA.
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Profiti G
Bologna Biocomputing Group, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
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Savojardo C
Bologna Biocomputing Group, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
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Martelli PL
Bologna Biocomputing Group, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
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Casadio R
Bologna Biocomputing Group, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
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Boecker F
University of Bonn: INRES Crop Bioinformatics, Bonn, North Rhine-Westphalia, Germany.
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Schoof H
INRES Crop Bioinformatics, University of Bonn, Bonn, Germany.
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Kahanda I
Gianforte School of Computing, Montana State University, Bozeman, Montana, USA.
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Thurlby N
University of Bristol, Computer Science, Bristol, Bristol, United Kingdom.
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McHardy AC
Computational Biology of Infection Research, Helmholtz Centre for Infection Research, Brunswick, Germany.
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Renaux A
Interuniversity Institute of Bioinformatics in Brussels, Université libre de Bruxelles - Vrije Universiteit Brussel, Brussels, Belgium.
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Saidi R
European Molecular Biolo gy Labora tory, European Bioinformatics Institute (EMBL-EBI), Cambridge, UK.
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Gough J
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
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Freitas AA
University of Kent, School of Computing, Canterbury, United Kingdom.
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Antczak M
School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom.
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Fabris F
University of Kent, School of Computing, Canterbury, United Kingdom.
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Wass MN
School of Biosciences, University of Kent, Canterbury, Kent, United Kingdom.
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Hou J
University of Missouri, Computer Science, Columbia, Missouri, USA.
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Cheng J
Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO, USA.
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Wang Z
University of Miami, Coral Gables, Florida, USA.
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Romero AE
Centre for Systems and Synthetic Biology, Department of Computer Science, Royal Holloway, University of London, Egham, Surrey, United Kingdom.
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Paccanaro A
Centre for Systems and Synthetic Biology, Department of Computer Science, Royal Holloway, University of London, Egham, Surrey, United Kingdom.
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Yang H
School of Mathematics, Statistics and Applied Mathematics, National University of Ireland, Galway, Galway, Ireland.
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Goldberg T
Department of Informatics, Bioinformatics & Computational Biology-i12, Technische Universitat Munchen, Munich, Germany.
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Zhao C
Faculty for Informatics, Garching, Germany.
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Holm L
Institute of Biotechnology, Helsinki Institute of Life Sciences, University of Helsinki, Finland, Helsinki, Finland.
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Törönen P
Institute of Biotechnology, Helsinki Institute of Life Sciences, University of Helsinki, Finland, Helsinki, Finland.
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Medlar AJ
Institute of Biotechnology, Helsinki Institute of Life Sciences, University of Helsinki, Finland, Helsinki, Finland.
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Zosa E
Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
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Borukhov I
Compugen Ltd., Holon, Israel.
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Novikov I
Baylor College of Medicine, Department of Biochemistry and Molecular Biology, Houston, TX, USA.
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Wilkins A
Baylor College of Medicine, Department of Molecular and Human Genetics, Houston, TX, USA.
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Lichtarge O
Baylor College of Medicine, Department of Molecular and Human Genetics, Houston, TX, USA.
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Chi PH
National TsingHua University, Hsinchu, Taiwan.
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Tseng WC
Department of Electrical Engineering in National Tsing Hua University, Hsinchu City, Taiwan.
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Linial M
The Hebrew University of Jerusalem, Jerusalem, Israel.
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Rose PW
University of California San Diego, San Diego Supercomputer Center, La Jolla, California, USA.
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Dessimoz C
Department of Computational Biology and Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
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Vidulin V
Department of Knowledge Technologies, Jozef Stefan Institute, Ljubljana, Slovenia.
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Dzeroski S
Jozef Stefan Institute, Ljubljana, Slovenia.
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Sillitoe I
Research Department of Structural and Molecular Biology, University College London, London, England.
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Das S
Research Department of Structural and Molecular Biology, University College London, London, United Kingdom.
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Lees JG
Research Department of Structural and Molecular Biology, University College London, London, United Kingdom.
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Jones DT
The Francis Crick Institute, Biomedical Data Science Laboratory, London, United Kingdom.
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Wan C
Department of Computer Science, University College London, London, United Kingdom.
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Cozzetto D
Department of Computer Science, University College London, London, United Kingdom.
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Fa R
Department of Computer Science, University College London, London, United Kingdom.
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Torres M
Centre for Systems and Synthetic Biology, Department of Computer Science, Royal Holloway, University of London, Egham, Surrey, United Kingdom.
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Warwick Vesztrocy A
Department of Genetics, Evolution and Environment, University College London, Gower Street, London, WC1E 6BT, United Kingdom.
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Rodriguez JM
Cardiovascular Proteomics Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
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Tress ML
Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
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Frasca M
Università degli Studi di Milano - Computer Science Department - AnacletoLab, Milan, Milan, Italy.
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Notaro M
Università degli Studi di Milano - Computer Science Department - AnacletoLab, Milan, Milan, Italy.
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Grossi G
Università degli Studi di Milano - Computer Science Department - AnacletoLab, Milan, Milan, Italy.
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Petrini A
Università degli Studi di Milano - Computer Science Department - AnacletoLab, Milan, Milan, Italy.
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Re M
Università degli Studi di Milano - Computer Science Department - AnacletoLab, Milan, Milan, Italy.
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Valentini G
Università degli Studi di Milano - Computer Science Department - AnacletoLab, Milan, Milan, Italy.
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Mesiti M
Università degli Studi di Milano - Computer Science Department - AnacletoLab, Milan, Milan, Italy.
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Roche DB
Department of Informatics, Bioinformatics and Computational Biology-i12, Technische Universitat Munchen, Munich, Germany.
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Reeb J
Department of Informatics, Bioinformatics and Computational Biology-i12, Technische Universitat Munchen, Munich, Germany.
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Ritchie DW
University of Lorraine, CNRS, Inria, LORIA, Nancy, 54000, France.
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Aridhi S
University of Lorraine, CNRS, Inria, LORIA, Nancy, 54000, France.
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Alborzi SZ
University of Lorraine, CNRS, Inria, LORIA, Nancy, 54000, France.
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Devignes MD
University of Lorraine, CNRS, Inria, LORIA, Nancy, 54000, France.
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Koo DCE
Department of Biology, New York University, New York, NY, USA.
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Bonneau R
NYU Center for Data Science, New York, 10010, NY, USA.
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Gligorijević V
Center for Computational Biology (CCB), Flatiron Institute, Simons Foundation, New York, New York, USA.
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Barot M
Center for Data Science, New York University, New York, 10011, NY, USA.
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Fang H
Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
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Toppo S
Department of Molecular Medicine, University of Padova, Padova, Italy.
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Lavezzo E
Department of Molecular Medicine, University of Padova, Padova, Italy.
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Falda M
Department of Biology, University of Padova, Padova, Italy.
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Berselli M
Department of Molecular Medicine, University of Padova, Padova, Italy.
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Tosatto SCE
CNR Institute of Neuroscience, Padova, Italy.
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Carraro M
Department of Biomedical Sciences, University of Padua, Padova, Italy.
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Piovesan D
Department of Biomedical Sciences, University of Padua, Padova, Italy.
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Ur Rehman H
Department of Computer Science, National University of Computer and Emerging Sciences, Peshawar, Khyber Pakhtoonkhwa, Pakistan.
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Mao Q
Department of Computer and Information Sciences, Temple University, Philadelphia, PA, USA.
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Zhang S
Department of Computer and Information Sciences, Temple University, Philadelphia, PA, USA.
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Vucetic S
Department of Computer and Information Sciences, Temple University, Philadelphia, PA, USA.
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Black GS
Department of Biology, Brigham Young University, Provo, UT, USA.
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Jo D
Department of Biology, Brigham Young University, Provo, UT, USA.
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Suh E
Department of Biology, Brigham Young University, Provo, UT, USA.
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Dayton JB
Department of Biology, Brigham Young University, Provo, UT, USA.
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Larsen DJ
Department of Biology, Brigham Young University, Provo, UT, USA.
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Omdahl AR
Department of Biology, Brigham Young University, Provo, UT, USA.
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McGuffin LJ
School of Biological Sciences, University of Reading, Reading, England, United Kingdom.
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Brackenridge DA
School of Biological Sciences, University of Reading, Reading, England, United Kingdom.
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Babbitt PC
Department of Pharmaceutical Chemistry, San Francisco, CA, USA.
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Yunes JM
UC Berkeley - UCSF Graduate Program in Bioengineering, University of California, San Francisco, 94158, CA, USA.
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Fontana P
Research and Innovation Center, Edmund Mach Foundation, San Michele all'Adige, Italy.
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Zhang F
State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Fudan University, Shanghai, Shanghai, China.
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Zhu S
School of Computer Science and Shanghai Key Lab of Intelligent Information Processing, Fudan University, Shanghai, China.
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You R
School of Computer Science and Shanghai Key Lab of Intelligent Information Processing, Fudan University, Shanghai, China.
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Zhang Z
School of Computer Science and Shanghai Key Lab of Intelligent Information Processing, Fudan University, Shanghai, China.
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Dai S
School of Computer Science and Shanghai Key Lab of Intelligent Information Processing, Fudan University, Shanghai, China.
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Yao S
School of Computer Science and Shanghai Key Lab of Intelligent Information Processing, Fudan University, Shanghai, China.
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Tian W
State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Department of Biostatistics and Computational Biology, School of Life Sciences, Fudan University, Shanghai, Shanghai, China.
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Cao R
Department of Computer Science, Pacific Lutheran University, Tacoma, WA, USA.
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Chandler C
Department of Computer Science, Pacific Lutheran University, Tacoma, WA, USA.
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Amezola M
Department of Computer Science, Pacific Lutheran University, Tacoma, WA, USA.
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Johnson D
Department of Computer Science, Pacific Lutheran University, Tacoma, WA, USA.
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Chang JM
Department of Computer Science, National Chengchi University, Taipei, Taiwan.
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Liao WH
Department of Computer Science, National Chengchi University, Taipei, Taiwan.
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Liu YW
Department of Computer Science, National Chengchi University, Taipei, Taiwan.
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Pascarelli S
Okinawa Institute of Science and Technology, Tancha, Okinawa, Japan.
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Frank Y
Tel Aviv University, Tel Aviv, Israel.
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Hoehndorf R
Computer, Electrical and Mathematical Sciences & Engineering Division, Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Jeddah, Saudi Arabia.
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Kulmanov M
Computer, Electrical and Mathematical Sciences & Engineering Division, Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Jeddah, Saudi Arabia.
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Boudellioua I
Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
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Politano G
Control and Computer Engineering Department, Politecnico di Torino, Torino, TO, Italy.
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Di Carlo S
Control and Computer Engineering Department, Politecnico di Torino, Torino, TO, Italy.
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Benso A
Control and Computer Engineering Department, Politecnico di Torino, Torino, TO, Italy.
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Hakala K
Department of Future Technologies, Turku NLP Group, University of Turku, Turku, Finland.
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Ginter F
Department of Future Technologies, Turku NLP Group, University of Turku, Turku, Finland.
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Mehryary F
Department of Future Technologies, Turku NLP Group, University of Turku, Turku, Finland.
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Kaewphan S
Department of Future Technologies, Turku NLP Group, University of Turku, Turku, Finland.
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Björne J
Department of Future Technologies, Faculty of Science and Engineering, University of Turku, Turku, FI-20014, Finland.
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Moen H
University of Turku, Turku, Finland.
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Tolvanen MEE
Department of Future Technologies, University of Turku, Turku, Finland.
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Salakoski T
Department of Future Technologies, Faculty of Science and Engineering, University of Turku, Turku, FI-20014, Finland.
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Kihara D
Department of Biological Sciences, Department of Computer Science, Purdue University, 47907, IN, USA.
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Jain A
Department of Computer Science, Purdue University, West Lafayette, IN, USA.
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Šmuc T
Division of Electronics, Rudjer Boskovic Institute, Zagreb, Croatia.
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Altenhoff A
Department of Computer Science, ETH Zurich, Zurich, Switzerland.
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Ben-Hur A
Department of Computer Science, Colorado State University, Fort Collins, CO, USA.
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Rost B
Department of Informatics, Bioinformatics & Computational Biology-i12, Technische Universitat Munchen, Munich, Germany.
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Brenner SE
University of California, Berkeley, CA, USA.
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Orengo CA
Research Department of Structural and Molecular Biology, University College London, London, United Kingdom.
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Jeffery CJ
Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, USA.
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Bosco G
Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
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Hogan DA
Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
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Martin MJ
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, United Kingdom.
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O'Donovan C
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, United Kingdom.
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Mooney SD
Department of Biomedical Informatics and Medical Education, University of Washington, Seattle, WA, USA.
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Greene CS
Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
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Radivojac P
Khoury College of Computer Sciences, Northeastern University, Boston, MA, USA. predrag@northeastern.edu.
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Friedberg I
Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA. idoerg@iastate.edu.
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English
BACKGROUND
The Critical Assessment of Functional Annotation (CAFA) is an ongoing, global, community-driven effort to evaluate and improve the computational annotation of protein function.
RESULTS
Here, we report on the results of the third CAFA challenge, CAFA3, that featured an expanded analysis over the previous CAFA rounds, both in terms of volume of data analyzed and the types of analysis performed. In a novel and major new development, computational predictions and assessment goals drove some of the experimental assays, resulting in new functional annotations for more than 1000 genes. Specifically, we performed experimental whole-genome mutation screening in Candida albicans and Pseudomonas aureginosa genomes, which provided us with genome-wide experimental data for genes associated with biofilm formation and motility. We further performed targeted assays on selected genes in Drosophila melanogaster, which we suspected of being involved in long-term memory.
CONCLUSION
We conclude that while predictions of the molecular function and biological process annotations have slightly improved over time, those of the cellular component have not. Term-centric prediction of experimental annotations remains equally challenging; although the performance of the top methods is significantly better than the expectations set by baseline methods in C. albicans and D. melanogaster, it leaves considerable room and need for improvement. Finally, we report that the CAFA community now involves a broad range of participants with expertise in bioinformatics, biological experimentation, biocuration, and bio-ontologies, working together to improve functional annotation, computational function prediction, and our ability to manage big data in the era of large experimental screens.
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Language
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Open access status
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gold
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Identifiers
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Persistent URL
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https://folia.unifr.ch/global/documents/221119
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