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Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.

  • Kanost MR Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA. Electronic address: Kanost@ksu.edu.
  • Arrese EL Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Cao X Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Chen YR Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY, 14853, USA.
  • Chellapilla S KSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
  • Goldsmith MR Biological Sciences Department, University of Rhode Island, Kingston, RI, 02881, USA.
  • Grosse-Wilde E Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
  • Heckel DG Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Herndon N KSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
  • Jiang H Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Papanicolaou A Hawkesbury Institute for the Environment, Western Sydney University, Richmond, NSW, 2753, Australia.
  • Qu J Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Soulages JL Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Vogel H Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Walters J Department of Ecology and Evolutionary Biology, Univ. Kansas, Lawrence, KS, 66045, USA.
  • Waterhouse RM Department of Genetic Medicine and Development, University of Geneva Medical School, rue Michel-Servet 1, 1211, Geneva, Switzerland; Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211, Geneva, Switzerland; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, 32 Vassar Street, Cambridge, MA, 02139, USA; The Broad Institute of MIT and Harvard, Cambridge, 415 Main Street, MA, 02142, USA.
  • Ahn SJ Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Almeida FC Departament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
  • An C Department of Entomology, China Agricultural University, Beijing, China.
  • Aqrawi P Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Bretschneider A Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Bryant WB Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
  • Bucks S Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
  • Chao H Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Chevignon G Institut de Recherche sur la Biologie de l'Insecte, UMR CNRS 7261, UFR Sciences et Techniques, Université François-Rabelais, Tours, France.
  • Christen JM Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
  • Clarke DF CSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
  • Dittmer NT Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
  • Ferguson LCF Department of Zoology, South Parks Road, Oxford, UK.
  • Garavelou S Centre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
  • Gordon KHJ CSIRO Health and Biosecurity, Clunies Ross St, Acton, ACT, 2601, Australia.
  • Gunaratna RT Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Han Y Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Hauser F Center for Functional and Comparative Insect Genomics, Department of Biology, University of Copenhagen, Universitetsparken 15, DK-21oo, Copenhagen, Denmark.
  • He Y Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Heidel-Fischer H Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Hirsh A Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
  • Hu Y Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Jiang H Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, China.
  • Kalra D Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Klinner C Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
  • König C Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
  • Kovar C Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Kroll AR Department of Biology, Reed College, Portland, OR, 97202, USA.
  • Kuwar SS Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Lee SL Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Lehman R Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Bioresources Project Group, Winchesterstrasse 2, 35394, Gießen, Germany.
  • Li K College of Chemistry, Chemical Engineering, and Biotechnology, Donghua University, Shanghai, 201620, China.
  • Li Z College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
  • Liang H McDermott Center for Human Growth and Development, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA.
  • Lovelace S Department of Biological Sciences, University of Southern Maine, Portland, ME, 04104, USA.
  • Lu Z College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
  • Mansfield JH Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
  • McCulloch KJ Department of Ecology and Evolutionary Biology, University of California, Irvine, CA, 92697, USA.
  • Mathew T Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Morton B Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
  • Muzny DM Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Neunemann D Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Ongeri F Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Pauchet Y Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
  • Pu LL Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Pyrousis I Centre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
  • Rao XJ School of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China.
  • Redding A Department of Biology, University of Rochester, Rochester, NY, 14627, USA.
  • Roesel C Department of Marine and Environmental Sciences, Northeastern University, Boston, MA, 02115, USA.
  • Sanchez-Gracia A Departament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
  • Schaack S Department of Biology, Reed College, Portland, OR, 97202, USA.
  • Shukla A Department of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
  • Tetreau G Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY, 14456, USA.
  • Wang Y Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Xiong GH State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • Traut W Institut fuer Biologie, Universitaet Luebeck, D-23538, Luebeck, Germany.
  • Walsh TK CSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
  • Worley KC Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Wu D Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
  • Wu W Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
  • Wu YQ Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Zhang X Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
  • Zou Z State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • Zucker H Neuroscience Program, Hamilton College, Clinton, NY, 13323, USA.
  • Briscoe AD Department of Ecology and Evolutionary Biology, University of California, Irvine, CA, 92697, USA.
  • Burmester T Institute of Zoology, University of Hamburg, Germany.
  • Clem RJ Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
  • Feyereisen R Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
  • Grimmelikhuijzen CJP Center for Functional and Comparative Insect Genomics, Department of Biology, University of Copenhagen, Universitetsparken 15, DK-21oo, Copenhagen, Denmark.
  • Hamodrakas SJ Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece.
  • Hansson BS Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
  • Huguet E Institut de Recherche sur la Biologie de l'Insecte, UMR CNRS 7261, UFR Sciences et Techniques, Université François-Rabelais, Tours, France.
  • Jermiin LS CSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
  • Lan Q Department of Entomology, University of Wisconsin, Madison, USA.
  • Lehman HK Biology Department and Neuroscience Program, Hamilton College, Clinton, NY, 13323, USA.
  • Lorenzen M Dept. Entomology, North Carolina State Univ., Raleigh, NC, 27695, USA.
  • Merzendorfer H University of Siegen, School of Natural Sciences and Engineering, Institute of Biology - Molecular Biology, Adolf-Reichwein-Strasse. 2, AR-C3010, 57076 Siegen, Germany.
  • Michalopoulos I Centre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
  • Morton DB Department of Integrative Biosciences, School of Dentistry, BRB421, L595, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd., Portland, OR, 97239, USA.
  • Muthukrishnan S Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
  • Oakeshott JG CSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
  • Palmer W Department of Genetics, University of Cambridge, Downing St, Cambridge, CB2 3EH, UK.
  • Park Y Department of Entomology, Kansas State University, Manhattan, KS, 66506, USA.
  • Passarelli AL Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
  • Rozas J Departament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
  • Schwartz LM Department of Biology, University of Massachusetts, Amherst, MA, 01003, USA.
  • Smith W Department of Biology, Northeastern University, Boston, MA, 02115, USA.
  • Southgate A Department of Biology, College of Charleston, Charleston, SC, 29424, USA.
  • Vilcinskas A Institute for Insect Biotechnology, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.
  • Vogt R Department of Biological Sciences, University of South Carolina, Columbia, SC, 29205, USA.
  • Wang P Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY, 14456, USA.
  • Werren J Department of Biology, University of Rochester, Rochester, NY, 14627, USA.
  • Yu XQ University of Missouri-Kansas City, 5007 Rockhill Road, Kansas City, MO, 64110, USA.
  • Zhou JJ Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
  • Brown SJ KSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
  • Scherer SE Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Richards S Human Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
  • Blissard GW Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY, 14853, USA.
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  • 2016-08-16
Published in:
  • Insect biochemistry and molecular biology. - 2016
Innate immunity
Insect
Insect biochemistry
Lepidoptera
Moth
Synteny
Tobacco hornworm
Animals
Gene Expression
Gene Expression Profiling
Genome, Insect
Larva
Manduca
Pupa
Sequence Analysis, DNA
Synteny
English Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/216177
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