Journal article
Intravoxel incoherent motion analysis of abdominal organs: computation of reference parameters in a large cohort of C57Bl/6 mice and correlation to microvessel density.
- Eberhardt C Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland. christian.eberhardt@usz.ch.
- Wurnig MC Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
- Wirsching A Department of Visceral and Transplant Surgery, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
- Rossi C Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
- Rottmar M Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
- Özbay PS Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
- Filli L Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
- Lesurtel M Swiss HPB and Transplant Center, University Hospital Zurich, Rämistrasse 100, 8091, Zurich, Switzerland.
- Boss A Department of Diagnostic and Interventional Radiology, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland.
- 2016-04-21
Published in:
- Magma (New York, N.Y.). - 2016
Diffusion-weighted magnetic resonance imaging
Echo-planar imaging
IVIM
Intravoxel incoherent motion
Microvessel count
Microvessel density
Pseudodiffusion
Abdomen
Animals
Diffusion
Diffusion Magnetic Resonance Imaging
Echo-Planar Imaging
Image Processing, Computer-Assisted
Immunohistochemistry
Intestine, Small
Kidney
Liver
Mice
Mice, Inbred C57BL
Microcirculation
Microvessels
Motion
Pancreas
Perfusion
Platelet Endothelial Cell Adhesion Molecule-1
Spleen
English
OBJECTIVE
Diffusion-weighted magnetic resonance imaging (DW-MRI) combined with intravoxel incoherent motion (IVIM) analysis may be applied for assessment of organ lesions, diffuse parenchymal pathologies, and therapy monitoring. The aim of this study was to determine IVIM reference parameters of abdominal organs for translational research in a large cohort of C57Bl/6 laboratory mice.
MATERIALS AND METHODS
Anesthetized mice (n = 29) were measured in a 4.7 T small-animal MR scanner with a diffusion-weighted echo-planar imaging sequence at the [Formula: see text]-values 0, 13, 24, 55, 107, 260, 514, 767, 1020 s/mm(2). IVIM analysis was conducted on the liver, spleen, renal medulla and cortex, pancreas, and small bowel with computation of the true tissue diffusion coefficient [Formula: see text], the perfusion fraction [Formula: see text], and the pseudodiffusion coefficient [Formula: see text]. Microvessel density (MVD) was assessed by immunohistochemistry (IHC) against panendothelial cell antigen CD31.
RESULTS
Mean values of the different organs [[Formula: see text] (10(-3) mm(2)/s); [Formula: see text] (%); [Formula: see text] (10(-3) mm(2)/s); MVD (MV/mm(2))]: liver 1.15 ± 0.14; 14.77 ± 6.15; 50.28 ± 33.21, 2008.48 ± 419.43, spleen 0.55 ± 0.12; 9.89 ± 5.69; 24.46 ± 17.31; n.d., renal medulla 1.50 ± 0.20; 14.63 ± 4.07; 35.50 ± 18.01; 1231.88 ± 290.61, renal cortex 1.34 ± 0.18; 10.83 ± 3.70; 16.74 ± 6.74; 810.09 ± 193.50, pancreas 1.23 ± 0.22; 20.12 ± 7.46; 29.35 ± 17.82, 591.15 ± 86.25 and small bowel 1.06 ± 0.13; 16.48 ± 3.63; 15.31 ± 7.00; 420.50 ± 168.42. Unlike [Formula: see text] and [Formula: see text], [Formula: see text] correlates significantly with MVD (r = 0.90, p = 0.037).
CONCLUSION
This systematic evaluation of murine abdominal organs with IVIM and MVD analysis allowed to establish reference parameters for future DW-MRI translational research studies on small-animal disease models.
Diffusion-weighted magnetic resonance imaging (DW-MRI) combined with intravoxel incoherent motion (IVIM) analysis may be applied for assessment of organ lesions, diffuse parenchymal pathologies, and therapy monitoring. The aim of this study was to determine IVIM reference parameters of abdominal organs for translational research in a large cohort of C57Bl/6 laboratory mice.
MATERIALS AND METHODS
Anesthetized mice (n = 29) were measured in a 4.7 T small-animal MR scanner with a diffusion-weighted echo-planar imaging sequence at the [Formula: see text]-values 0, 13, 24, 55, 107, 260, 514, 767, 1020 s/mm(2). IVIM analysis was conducted on the liver, spleen, renal medulla and cortex, pancreas, and small bowel with computation of the true tissue diffusion coefficient [Formula: see text], the perfusion fraction [Formula: see text], and the pseudodiffusion coefficient [Formula: see text]. Microvessel density (MVD) was assessed by immunohistochemistry (IHC) against panendothelial cell antigen CD31.
RESULTS
Mean values of the different organs [[Formula: see text] (10(-3) mm(2)/s); [Formula: see text] (%); [Formula: see text] (10(-3) mm(2)/s); MVD (MV/mm(2))]: liver 1.15 ± 0.14; 14.77 ± 6.15; 50.28 ± 33.21, 2008.48 ± 419.43, spleen 0.55 ± 0.12; 9.89 ± 5.69; 24.46 ± 17.31; n.d., renal medulla 1.50 ± 0.20; 14.63 ± 4.07; 35.50 ± 18.01; 1231.88 ± 290.61, renal cortex 1.34 ± 0.18; 10.83 ± 3.70; 16.74 ± 6.74; 810.09 ± 193.50, pancreas 1.23 ± 0.22; 20.12 ± 7.46; 29.35 ± 17.82, 591.15 ± 86.25 and small bowel 1.06 ± 0.13; 16.48 ± 3.63; 15.31 ± 7.00; 420.50 ± 168.42. Unlike [Formula: see text] and [Formula: see text], [Formula: see text] correlates significantly with MVD (r = 0.90, p = 0.037).
CONCLUSION
This systematic evaluation of murine abdominal organs with IVIM and MVD analysis allowed to establish reference parameters for future DW-MRI translational research studies on small-animal disease models.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1007/s10334-016-0540-9
- PMID 27094553
- Persistent URL
- https://folia.unifr.ch/global/documents/212065
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