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Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro.
Journal article

Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro.

  • Hesler M Fraunhofer Institute for Biomedical Engineering IBMT, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany. Electronic address: michelle.hesler@ibmt.fraunhofer.de.
  • Aengenheister L Particles-Biology Interactions, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland. Electronic address: leonie.aengenheister@empa.ch.
  • Ellinger B Branch Lab ScreeningPort of the Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Schnackenburgallee 114, 22525 Hamburg, Germany. Electronic address: bernhard.ellinger@ime.fraunhofer.de.
  • Drexel R Postnova Analytics GmbH, Max-Planck-Straße 14, 86899 Landsberg am Lech, Germany. Electronic address: roland.drexel@postnova.com.
  • Straskraba S Goethe University Frankfurt, Institute for Molecular Bioscience, Max-von-Laue-Straße 9, 60438 Frankfurt am Main, Germany. Electronic address: susanne.straskraba@bio.uni-frankfurt.de.
  • Jost C PlasmaChem GmbH, Schwarzschildstraße 10, 12489 Berlin, Germany. Electronic address: c.jost@plasmachem.com.
  • Wagner S Fraunhofer Institute for Biomedical Engineering IBMT, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany. Electronic address: sylvia.wagner@ibmt.fraunhofer.de.
  • Meier F Postnova Analytics GmbH, Max-Planck-Straße 14, 86899 Landsberg am Lech, Germany. Electronic address: florian.meier@postnova.com.
  • von Briesen H Fraunhofer Institute for Biomedical Engineering IBMT, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany. Electronic address: hagen.briesen@ibmt.fraunhofer.de.
  • Büchel C Goethe University Frankfurt, Institute for Molecular Bioscience, Max-von-Laue-Straße 9, 60438 Frankfurt am Main, Germany.
  • Wick P Particles-Biology Interactions, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland. Electronic address: peter.wick@empa.ch.
  • Buerki-Thurnherr T Particles-Biology Interactions, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland. Electronic address: tina.buerki@empa.ch.
  • Kohl Y Fraunhofer Institute for Biomedical Engineering IBMT, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany. Electronic address: yvonne.kohl@ibmt.fraunhofer.de.
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  • 2019-07-31
Published in:
  • Toxicology in vitro : an international journal published in association with BIBRA. - 2019
Embryotoxicity
Intestinal barrier
Multi-endpoint toxicity study
Nano- and microplastics
Placental barrier
Polystyrene
Animals
Biological Transport
Cell Differentiation
Cell Line
Cell Survival
Female
Humans
Intestinal Mucosa
Mice
Micronucleus Tests
Models, Biological
Nanoparticles
Particle Size
Placenta
Polystyrenes
Pregnancy
English Nanoplastics (NP) and microplastics (MP) accumulate in our environment as a consequence of the massive consumption of plastics. Huge knowledge-gaps exist regarding uptake and fate of plastic particles in micro- and nano-dimensions in humans as well as on their impact on human health. This study investigated the transport and effects of 50 nm and 0.5 μm COOH-modified polystyrene (PS) particles, as representatives for NP and MP, in different biological models in vitro. Acute toxicity and potential translocation of the particles were studied at the human intestinal and placental barrier using advanced in vitro co-culture models. Furthermore, embryotoxicity and genotoxicity were investigated as highly sensitive endpoints. Polystyrene was not acutely toxic in both sizes (nano- and microparticles). No transport across the intestinal and placental barrier but a cellular uptake and intracellular accumulation of PS nano- and microparticles were determined. The particles were identified as weak embryotoxic and non-genotoxic. In contrast to single-organ studies, this multi-endpoint study is providing a data-set with the exact same type of particles to compare organ-specific outcomes. Our study clearly shows the need to investigate other types of plastics as well as towards long-term or chronic effects of plastic particles in different biological models in vitro.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/210295
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