Back
Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy.
Journal article

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy.

Show more…
  • 2013-03-15
Published in:
  • Journal of biomedical optics. - 2013
Animals
CHO Cells
Cell Membrane Permeability
Cell Size
Cells, Cultured
Cricetinae
Cricetulus
Glutamic Acid
HEK293 Cells
Holography
Humans
Intracellular Space
Mice
Microscopy, Phase-Contrast
Osmosis
Osmotic Pressure
Refractometry
Reproducibility of Results
Signal Processing, Computer-Assisted
Water
English A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When adding the absorbing dye fast green FCF as a dispersive agent to the extracellular medium, cellular thickness can be univocally determined in the full field of view. In addition to the absolute cell volume, the method can be applied to derive important biophysical parameters of living cells including osmotic membrane water permeability coefficient and the integral intracellular refractive index (RI). Further, the RI of transmembrane flux can be determined giving an indication about the nature of transported solutes. The proposed method is applied to cultured human embryonic kidney cells, Chinese hamster ovary cells, human red blood cells, mouse cortical astrocytes, and neurons.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/209732
Statistics
Document views: 7 File downloads: