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Sequential lignin depolymerization by combination of biocatalytic and formic acid/formate treatment steps.
Journal article

Sequential lignin depolymerization by combination of biocatalytic and formic acid/formate treatment steps.

  • Gasser CA Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland. christoph.gasser@fhnw.ch.
  • Čvančarová M Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
  • Ammann EM Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
  • Schäffer A Institute for Environmental Research, RWTH Aachen, Worringerweg 1, 52074, Aachen, Germany.
  • Shahgaldian P Institute for Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
  • Corvini PF Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
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  • 2016-12-02
Published in:
  • Applied microbiology and biotechnology. - 2017
Biocatalysis
Depolymerization
Formic acid
Laccase
Lignin
Acetates
Adsorption
Biocatalysis
Biomass
Enzymes, Immobilized
Factor Analysis, Statistical
Formates
Fungal Proteins
Laccase
Lignin
Magnetite Nanoparticles
Oxidation-Reduction
Triazoles
English Lignin, a complex three-dimensional amorphous polymer, is considered to be a potential natural renewable resource for the production of low-molecular-weight aromatic compounds. In the present study, a novel sequential lignin treatment method consisting of a biocatalytic oxidation step followed by a formic acid-induced lignin depolymerization step was developed and optimized using response surface methodology. The biocatalytic step employed a laccase mediator system using the redox mediator 1-hydroxybenzotriazole. Laccases were immobilized on superparamagnetic nanoparticles using a sorption-assisted surface conjugation method allowing easy separation and reuse of the biocatalysts after treatment. Under optimized conditions, as much as 45 wt% of lignin could be solubilized either in aqueous solution after the first treatment or in ethyl acetate after the second (chemical) treatment. The solubilized products were found to be mainly low-molecular-weight aromatic monomers and oligomers. The process might be used for the production of low-molecular-weight soluble aromatic products that can be purified and/or upgraded applying further downstream processes.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/208821
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