TECPR2 Cooperates with LC3C to Regulate COPII-Dependent ER Export.
- Stadel D Institute of Biochemistry II, Medical School Goethe University, 60590 Frankfurt, Germany.
- Millarte V Department of Biology, University of Konstanz, 78464 Konstanz, Germany; Biotechnology Institute Thurgau, 8280 Kreuzlingen, Switzerland.
- Tillmann KD Department of Biology, University of Konstanz, 78464 Konstanz, Germany; Biotechnology Institute Thurgau, 8280 Kreuzlingen, Switzerland.
- Huber J Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany.
- Tamin-Yecheskel BC Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel.
- Akutsu M Institute of Biochemistry II, Medical School Goethe University, 60590 Frankfurt, Germany; Buchmann Institute for Molecular Life Sciences, Goethe University, 60438 Frankfurt, Germany.
- Demishtein A Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel.
- Ben-Zeev B Edmond and Lilly Safra Children's Hospital, Sheba Medical Center, Ramat Gan, 52621, Israel; Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 69978, Israel.
- Anikster Y Edmond and Lilly Safra Children's Hospital, Sheba Medical Center, Ramat Gan, 52621, Israel; Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 69978, Israel.
- Perez F Institute Curie, CNRS UMR144, Paris, 75248, France.
- Dötsch V Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany.
- Elazar Z Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel.
- Rogov V Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany.
- Farhan H Department of Biology, University of Konstanz, 78464 Konstanz, Germany; Biotechnology Institute Thurgau, 8280 Kreuzlingen, Switzerland.
- Behrends C Institute of Biochemistry II, Medical School Goethe University, 60590 Frankfurt, Germany. Electronic address: behrends@em.uni-frankfurt.de.
- 2015-10-03
Published in:
- Molecular cell. - 2015
Adaptor Proteins, Signal Transducing
Autophagy
Carrier Proteins
Endoplasmic Reticulum
HeLa Cells
Humans
Microtubule-Associated Proteins
Mutation
Nerve Tissue Proteins
Protein Transport
Spastic Paraplegia, Hereditary
Vesicular Transport Proteins
English
Hereditary spastic paraplegias (HSPs) are a diverse group of neurodegenerative diseases that are characterized by axonopathy of the corticospinal motor neurons. A mutation in the gene encoding for Tectonin β-propeller containing protein 2 (TECPR2) causes HSP that is complicated by neurological symptoms. While TECPR2 is a human ATG8 binding protein and positive regulator of autophagy, the exact function of TECPR2 is unknown. Here, we show that TECPR2 associates with several trafficking components, among them the COPII coat protein SEC24D. TECPR2 is required for stabilization of SEC24D protein levels, maintenance of functional ER exit sites (ERES), and efficient ER export in a manner dependent on binding to lipidated LC3C. TECPR2-deficient HSP patient cells display alterations in SEC24D abundance and ER export efficiency. Additionally, TECPR2 and LC3C are required for autophagosome formation, possibly through maintaining functional ERES. Collectively, these results reveal that TECPR2 functions as molecular scaffold linking early secretion pathway and autophagy.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1016/j.molcel.2015.09.010
- PMID 26431026
- Persistent URL
- https://folia.unifr.ch/global/documents/208791
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