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Hydrogen sulfide measurement by headspace-gas chromatography-mass spectrometry (HS-GC-MS): application to gaseous samples and gas dissolved in muscle.

  • Varlet V Forensic Toxicology and Chemistry Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland vincent.varlet@chuv.ch.
  • Giuliani N Forensic Toxicology and Chemistry Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland.
  • Palmiere C Forensic Medicine Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland.
  • Maujean G Institut Universitaire de Médecine Légale, 12 Avenue Rockefeller, 69005 Lyon, France.
  • Augsburger M Forensic Toxicology and Chemistry Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland.
  • 2014-10-23
Published in:
  • Journal of analytical toxicology. - 2015
Adult
Autopsy
Calibration
Chemical Fractionation
Gas Chromatography-Mass Spectrometry
Humans
Hydrogen Sulfide
Male
Muscle, Skeletal
Reproducibility of Results
Sensitivity and Specificity
Specimen Handling
Sulfides
English The aim of our study was to present a new headspace-gas chromatography-mass spectrometry (HS-GC-MS) method applicable to the routine determination of hydrogen sulfide (H(2)S) concentrations in biological and gaseous samples. The primary analytical drawback of the GC/MS methods for H(2)S measurement discussed in the literature was the absence of a specific H(2)S internal standard required to perform quantification. Although a deuterated hydrogen sulfide (D(2)S) standard is currently available, this standard is not often used because this standard is expensive and is only available in the gas phase. As an alternative approach, D(2)S can be generated in situ by reacting deuterated chloride with sodium sulfide; however, this technique can lead to low recovery yield and potential isotopic fractionation. Therefore, N(2)O was chosen for use as an internal standard. This method allows precise measurements of H(2)S concentrations in biological and gaseous samples. Therefore, a full validation using accuracy profile based on the β-expectation tolerance interval is presented. Finally, this method was applied to quantify H(2)S in an actual case of H(2)S fatal intoxication.
Language
  • English
Open access status
bronze
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Persistent URL
https://folia.unifr.ch/global/documents/20299
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