Echinococcus multilocularis: immunological study on the "Em2-positive" laminated layer during in vitro and in vivo post-oncospheral and larval development.
- Gottstein B Institute of Parasitology, University of Zürich, Switzerland.
- Deplazes P
- Aubert M
- 1992-01-01
Published in:
- Parasitology research. - 1992
Animals
Antibodies, Helminth
Antigens, Helminth
Echinococcosis
Echinococcus
Fluorescent Antibody Technique
Larva
Mice
Mice, Inbred C57BL
English
Echinococcus multilocularis oncospheres, primary vesicular cysts, and protoscolices were assessed in vitro and in vivo for their potential to synthesize a PAS-positive laminated layer containing monoclonal antibody (mAb) G11-binding Em2 antigen. The presence of Em2 antigen in developed oncospheres and cysts was subsequently correlated to the potential of in vivo development into a secondary metacestode in recipient host mice, which also responded by anti-Em2 serum antibody formation. In contrast, protoscolices failed to develop the "Em2-positive" layer in vitro under the selected experimental conditions. The failure to develop subsequently in vivo into a secondary metacestode was underlined by a lack of anti-Em2 serum antibody formation by the hosts. We furthermore developed a technique to obtain E. multilocularis clones by inoculating single oncospheres into recipient mice.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/bf00937086
- PMID 1409528
- Persistent URL
- https://folia.unifr.ch/global/documents/199853
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