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Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions
Journal article

Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions

  • Prigge, Michael J ORCID Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United States
  • Platre, Matthieu Plant Molecular and Cellular Biology Laboratory and Integrative Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States
  • Kadakia, Nikita Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United States
  • Zhang, Yi Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United States
  • Greenham, Kathleen ORCID Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United States
  • Szutu, Whitnie ORCID Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United States
  • Pandey, Bipin Kumar ORCID Plant and Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, United Kingdom
  • Bhosale, Rahul Arvind ORCID Plant and Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, United Kingdom
  • Bennett, Malcolm J Plant and Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, United Kingdom
  • Busch, Wolfgang Plant Molecular and Cellular Biology Laboratory and Integrative Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States
  • Estelle, Mark ORCID Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United States
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  • 2020-2-18
Published in:
  • eLife. - eLife Sciences Publications, Ltd. - 2020, vol. 9
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience
General Medicine
English The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/196619
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