Back
Full-text
Journal article

Temporal patterning of apical progenitors and their daughter neurons in the developing neocortex.

  • Telley L Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland. ludovic.telley@unil.ch denis.jabaudon@unige.ch.
  • Agirman G Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Prados J Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Amberg N Institute of Science and Technology Austria, Klosterneuburg, Austria.
  • Fièvre S Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Oberst P Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Bartolini G Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Vitali I Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Cadilhac C Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Hippenmeyer S Institute of Science and Technology Austria, Klosterneuburg, Austria.
  • Nguyen L GIGA-Stem Cells, University of Liège, C.H.U. Sart Tilman, Liège, Belgium.
  • Dayer A Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
  • Jabaudon D Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland. ludovic.telley@unil.ch denis.jabaudon@unige.ch.
Show more…
  • 2019-05-11
Published in:
  • Science (New York, N.Y.). - 2019
Animals
Epigenesis, Genetic
Gene Expression Regulation, Developmental
Mice
Neocortex
Neoplastic Stem Cells
Neurogenesis
Neurons
Polycomb Repressive Complex 2
RNA, Messenger
Sequence Analysis, RNA
Single-Cell Analysis
Transcription, Genetic
English During corticogenesis, distinct subtypes of neurons are sequentially born from ventricular zone progenitors. How these cells are molecularly temporally patterned is poorly understood. We used single-cell RNA sequencing at high temporal resolution to trace the lineage of the molecular identities of successive generations of apical progenitors (APs) and their daughter neurons in mouse embryos. We identified a core set of evolutionarily conserved, temporally patterned genes that drive APs from internally driven to more exteroceptive states. We found that the Polycomb repressor complex 2 (PRC2) epigenetically regulates AP temporal progression. Embryonic age-dependent AP molecular states are transmitted to their progeny as successive ground states, onto which essentially conserved early postmitotic differentiation programs are applied, and are complemented by later-occurring environment-dependent signals. Thus, epigenetically regulated temporal molecular birthmarks present in progenitors act in their postmitotic progeny to seed adult neuronal diversity.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/194727
Statistics
Document views: 34 File downloads:
  • Full-text: 0