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Tracking individual action potentials throughout mammalian axonal arbors.
Journal article

Tracking individual action potentials throughout mammalian axonal arbors.

  • Radivojevic M Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
  • Franke F Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
  • Altermatt M Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
  • Müller J Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
  • Hierlemann A Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
  • Bakkum DJ Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
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  • 2017-10-10
Published in:
  • eLife. - 2017
HD-MEA
axonal physiology
electrode-neuron interface
neuroscience
rat
Action Potentials
Animals
Cells, Cultured
Intravital Microscopy
Microelectrodes
Neurons
Rats, Wistar
English Axons are neuronal processes specialized for conduction of action potentials (APs). The timing and temporal precision of APs when they reach each of the synapses are fundamentally important for information processing in the brain. Due to small diameters of axons, direct recording of single AP transmission is challenging. Consequently, most knowledge about axonal conductance derives from modeling studies or indirect measurements. We demonstrate a method to noninvasively and directly record individual APs propagating along millimeter-length axonal arbors in cortical cultures with hundreds of microelectrodes at microsecond temporal resolution. We find that cortical axons conduct single APs with high temporal precision (~100 µs arrival time jitter per mm length) and reliability: in more than 8,000,000 recorded APs, we did not observe any conduction or branch-point failures. Upon high-frequency stimulation at 100 Hz, successive became slower, and their arrival time precision decreased by 20% and 12% for the 100th AP, respectively.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/184019
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