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Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.
Journal article

Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.

  • Cholley P Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
  • Stojanov M Service of Hospital Preventive Medicine, Lausanne University Hospital, Lausanne, Switzerland.
  • Hocquet D Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
  • Thouverez M Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
  • Bertrand X Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
  • Blanc DS Service of Hospital Preventive Medicine, Lausanne University Hospital, Lausanne, Switzerland. Electronic address: Dominique.Blanc@chuv.ch.
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  • 2015-05-09
Published in:
  • Diagnostic microbiology and infectious disease. - 2015
MLST
Molecular typing
PFGE
Pseudomonas aeruginosa
Pulsed field gel electrophoresis
Sequence typing
Electrophoresis, Gel, Pulsed-Field
Humans
Molecular Epidemiology
Molecular Typing
Pseudomonas Infections
Pseudomonas aeruginosa
Sequence Analysis, DNA
English Reliable molecular typing methods are necessary to investigate the epidemiology of bacterial pathogens. Reference methods such as multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) are costly and time consuming. Here, we compared our newly developed double-locus sequence typing (DLST) method for Pseudomonas aeruginosa to MLST and PFGE on a collection of 281 isolates. DLST was as discriminatory as MLST and was able to recognize "high-risk" epidemic clones. Both methods were highly congruent. Not surprisingly, a higher discriminatory power was observed with PFGE. In conclusion, being a simple method (single-strand sequencing of only 2 loci), DLST is valuable as a first-line typing tool for epidemiological investigations of P. aeruginosa. Coupled to a more discriminant method like PFGE or whole genome sequencing, it might represent an efficient typing strategy to investigate or prevent outbreaks.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/181595
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