Identification of Borrelia protein candidates in mouse skin for potential diagnosis of disseminated Lyme borreliosis.
- Grillon A EA7290, Virulence bactérienne précoce, groupe Borréliose de Lyme, Facultés de Médecine et de Pharmacie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, F-67000, Strasbourg, France.
- Westermann B Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, F-67000, Strasbourg, France.
- Cantero P Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, F-67000, Strasbourg, France.
- Jaulhac B EA7290, Virulence bactérienne précoce, groupe Borréliose de Lyme, Facultés de Médecine et de Pharmacie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, F-67000, Strasbourg, France.
- Voordouw MJ Laboratory of Ecology and Evolution of Parasites, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
- Kapps D EA7290, Virulence bactérienne précoce, groupe Borréliose de Lyme, Facultés de Médecine et de Pharmacie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, F-67000, Strasbourg, France.
- Collin E EA7290, Virulence bactérienne précoce, groupe Borréliose de Lyme, Facultés de Médecine et de Pharmacie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, F-67000, Strasbourg, France.
- Barthel C EA7290, Virulence bactérienne précoce, groupe Borréliose de Lyme, Facultés de Médecine et de Pharmacie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, F-67000, Strasbourg, France.
- Ehret-Sabatier L Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, F-67000, Strasbourg, France.
- Boulanger N EA7290, Virulence bactérienne précoce, groupe Borréliose de Lyme, Facultés de Médecine et de Pharmacie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, F-67000, Strasbourg, France. nboulanger@unistra.fr.
- 2017-12-03
Published in:
- Scientific reports. - 2017
Adrenal Cortex Hormones
Animals
Bacterial Proteins
Borrelia
Chromatography, High Pressure Liquid
DNA, Bacterial
Female
Flagellin
Ixodes
Lyme Disease
Mice
Mice, Inbred C3H
Peptides
Real-Time Polymerase Chain Reaction
Skin
Tandem Mass Spectrometry
English
In vector-borne diseases, the skin plays an essential role in the transmission of vector-borne pathogens between the vertebrate host and blood-feeding arthropods and in pathogen persistence. Borrelia burgdorferi sensu lato is a tick-borne bacterium that causes Lyme borreliosis (LB) in humans. This pathogen may establish a long-lasting infection in its natural vertebrate host where it can persist in the skin and some other organs. Using a mouse model, we demonstrate that Borrelia targets the skin regardless of the route of inoculation, and can persist there at low densities that are difficult to detect via qPCR, but that were infective for blood-feeding ticks. Application of immunosuppressive dermocorticoids at 40 days post-infection (PI) significantly enhanced the Borrelia population size in the mouse skin. We used non-targeted (Ge-LC-MS/MS) and targeted (SRM-MS) proteomics to detect several Borrelia-specific proteins in the mouse skin at 40 days PI. Detected Borrelia proteins included flagellin, VlsE and GAPDH. An important problem in LB is the lack of diagnosis methods capable of detecting active infection in humans suffering from disseminated LB. The identification of Borrelia proteins in skin biopsies may provide new approaches for assessing active infection in disseminated manifestations.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41598-017-16749-9
- PMID 29196626
- Persistent URL
- https://folia.unifr.ch/global/documents/172948
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