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Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry.
Journal article

Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry.

  • D'Atri V School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU - Rue Michel Servet 1, 1211 Geneva 4, Switzerland. Electronic address: valentina.datri@unige.ch.
  • Fekete S School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU - Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
  • Stoll D Department of Chemistry, Gustavus Adolphus College, Saint Peter, Minnesota 56082, United States.
  • Lauber M Waters Corporation, 34 Maple Street, Milford, Massachusetts 01757-3696, United States.
  • Beck A Center of Immunology Pierre Fabre, 5 Avenue Napoléon III, BP 60497, Saint-Julien-en-Genevois, France.
  • Guillarme D School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU - Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
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  • 2018-02-25
Published in:
  • Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. - 2018
Antibody-drug conjugate
Glycans profiling
High-resolution mass spectrometry
Hydrophilic interaction chromatography
Post-translational modifications
Brentuximab Vedotin
Chromatography, Liquid
Hydrophobic and Hydrophilic Interactions
Immunoconjugates
Mass Spectrometry
Polysaccharides
Protein Processing, Post-Translational
English Brentuximab vedotin (Adcetris) is a cysteine-linked antibody-drug conjugate (ADC) used in the treatment of Hodgkin lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). In this study, the drug payload and glycan modifications of this ADC were simultaneously characterized using a unique LC-MS middle-up analysis, involving hydrophilic interaction chromatography (HILIC). This work demonstrates that HILIC is an effective and complementary analytical technique to reversed phase liquid chromatography (RPLC) for subunit-level characterization of immuno-conjugates.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/170299
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