Back
Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability.
Journal article

Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability.

  • Chan LY Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
  • Mugler CF Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
  • Heinrich S Department of Biochemistry, ETH Zurich, Zurich, Switzerland.
  • Vallotton P Department of Biochemistry, ETH Zurich, Zurich, Switzerland.
  • Weis K Department of Biochemistry, ETH Zurich, Zurich, Switzerland.
Show more…
  • 2018-09-08
Published in:
  • eLife. - 2018
S. cerevisiae
chromosomes
decay
gene expression
genetics
genomics
mRNA
translation
Biotin
Cell Proliferation
Computer Simulation
Half-Life
Kinetics
Protein Biosynthesis
RNA Stability
RNA, Messenger
Reproducibility of Results
Ribosomes
Saccharomyces cerevisiae
Staining and Labeling
Transcriptome
English The cytoplasmic abundance of mRNAs is strictly controlled through a balance of production and degradation. Whereas the control of mRNA synthesis through transcription has been well characterized, less is known about the regulation of mRNA turnover, and a consensus model explaining the wide variations in mRNA decay rates remains elusive. Here, we combine non-invasive transcriptome-wide mRNA production and stability measurements with selective and acute perturbations to demonstrate that mRNA degradation is tightly coupled to the regulation of translation, and that a competition between translation initiation and mRNA decay -but not codon optimality or elongation- is the major determinant of mRNA stability in yeast. Our refined measurements also reveal a remarkably dynamic transcriptome with an average mRNA half-life of only 4.8 min - much shorter than previously thought. Furthermore, global mRNA destabilization by inhibition of translation initiation induces a dose-dependent formation of processing bodies in which mRNAs can decay over time.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://folia.unifr.ch/global/documents/168302
Statistics
Document views: 25 File downloads: